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Literature summary extracted from

  • Casey, P.J.; Rando, R.R.
    Isoprenylated protein peptidase Rce1 (2004), Handbook of Proteolytic Enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. )Academic Press, 2, 2123-2127.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.4.99.B1 medicine excision of RCE1 from skin carcinoma cell line retards the growth of cells significantly, and this effect was exaggerated by co-treatment with farnesyl-transferase inhibitor Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
3.4.99.B1 additional information RCE1 deficient mice generated, loss of the enzyme results in late embryonic lethality Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.99.B1 beta-(2-naphthyl)-Ala-CH2Cl affinity labeling agent of the enzyme Bos taurus
3.4.99.B1 additional information not inhibited by chyostatin, DTT, E-64, 1,10-phenanthroline and PMSF, Tos-Lys-CH2Cl Bos taurus
3.4.99.B1 N-Boc-S-farnesyl-L-cys-CH2Cl
-
 Bos taurus
3.4.99.B1 N-Boc-S-farnesyl-L-cysteine aldehyde potent competetive inhibitor Bos taurus
3.4.99.B1 p-chloromercuribenzoate inactivates the partially purified enzyme, with 0.005 mM substantial inhibition Bos taurus
3.4.99.B1 pseudo peptide R (CH2-NH)
-
 Bos taurus
3.4.99.B1 Tos-Phe-CH2Cl affinity labeling agent of the enzyme Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.99.B1 0.0057
-
 N-acetyl-S-farnesyl-L-cysteinyl-L-valyl-L-isoleucyl-L-serine
-
 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.99.B1 membrane
-
 Homo sapiens 16020
-
3.4.99.B1 microsome
-
 Bos taurus
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.4.99.B1 Bos taurus
-
-
-
3.4.99.B1 Homo sapiens
-
-
-
3.4.99.B1 Mus musculus
-
-
-
3.4.99.B1 Saccharomyces cerevisiae
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.99.B1 carcinoma cell line from skin Mus musculus
-
3.4.99.B1 liver
-
 Bos taurus
-
3.4.99.B1 MEF cell
-
 Mus musculus
-
3.4.99.B1 additional information Sf9 cell Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.99.B1 0.00000251
-
 with N-acetyl-S-farnesyl-Cys+Val-Ile-Ser as substrate Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.99.B1 a-factor-CaaX + H2O CaaX proteolysis of all three forms of Ras and other CaaX proteins is absent in RCE1 deficient mice, RCE1 is required for correct localization of these proteins Mus musculus a-factor-C + aaX
-
 ?
3.4.99.B1 N-acetyl-S-farnesyl-Cys-Val-Ile-Ser + H2O
-
 Bos taurus N-acetyl-S-farnesyl-Cys + Val-Ile-Ser
-
 ?
3.4.99.B1 N-Boc-S-farnesyl-L-Cys-Val-Ile-Met + H2O
-
 Bos taurus N-Boc-S-farnesyl-L-Cys + Val-Ile-Met
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.99.B1 isoprenylated protein peptidase
-
 Mus musculus
3.4.99.B1 isoprenylated protein peptidase
-
 Homo sapiens
3.4.99.B1 isoprenylated protein peptidase
-
 Saccharomyces cerevisiae
3.4.99.B1 isoprenylated protein peptidase
-
 Bos taurus
3.4.99.B1 RCE1
-
 Mus musculus
3.4.99.B1 RCE1
-
 Homo sapiens
3.4.99.B1 RCE1
-
 Saccharomyces cerevisiae
3.4.99.B1 RCE1
-
 Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.99.B1 7
-
-
 Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.99.B1 0.000085
-
 pseudo peptide R (CH2-NH)
-
 Bos taurus
3.4.99.B1 0.002
-
 N-Boc-S-farnesyl-L-cysteine aldehyde
-
 Bos taurus
3.4.99.B1 0.03
-
 N-Boc-S-farnesyl-L-cys-CH2Cl
-
 Bos taurus