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Literature summary extracted from

  • Lopez, V.; Alarcon, R.; Orellana, M.S.; Enriquez, P.; Uribe, E.; Martinez, J.; Carvajal, N.
    Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies. Alteration of substrate specificity by replacement of Asn149 with Asp (2005), FEBS J., 272, 4540-4548.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.5.3.1 H120N 53% of wild-type activity, no alteration in KM-value Homo sapiens
3.5.3.1 H145N 95% of wild-type activity, no alteration in KM-value Homo sapiens
3.5.3.1 N149D almost complete loss of activity with L-arginine, gain of activity with agmatine Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.3.1 1.4
-
 L-arginine 37°C, pH 9.0, wild-type Homo sapiens
3.5.3.1 1.5
-
 L-arginine 37°C, pH 9.0, mutant H145N Homo sapiens
3.5.3.1 1.6
-
 L-arginine 37°C, pH 9.0, mutant H120N Homo sapiens
3.5.3.1 2.5
-
 agmatine 37°C, pH 9.0, mutant N149D Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.3.1 Mn2+ activation. after dialysis with EDTA, wild-type and mutant H145N contain 1.1 and 1.3 Mn2+ per subunit, resp., and are hlaf-.active. Mutant H120N contains less than 0.1 Mn2+ per subunit and is inactive Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.1 Homo sapiens
-
 isoform arginase II
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.1 agmatine + H2O for wild-type, no substrate. Hydrolysis by mutant N149D Homo sapiens 1,4-diaminobutane + urea
-
 ?
3.5.3.1 L-arginine + H2O
-
 Homo sapiens L-ornithine + urea
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.3.1 131
-
 L-arginine 37°C, pH 9.0, mutant H120N Homo sapiens
3.5.3.1 238
-
 L-arginine 37°C, pH 9.0, mutant H145N Homo sapiens
3.5.3.1 249
-
 L-arginine 37°C, pH 9.0, wild-type Homo sapiens