EC Number | Cloned (Comment) | Organism |
---|---|---|
3.5.1.41 | expression of the C-terminally His6-tagged enzyme in Pichia pastoris and in Escherichia coli, the His-tag causes intramolecular interactions with the active site | Colletotrichum lindemuthianum |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.5.1.41 | purified recombinant His6-tagged enzyme expressed in Pichia pastoris, sitting drop vapour diffusion method, 20 mg/ml protein in 30 mM Tris-HCl, pH 8.0, is mixed with an equal volume of reservoir solution containing 30% w/v PEG 4000, 0.2 M ammonium acetate, and 0.1 M sodium acetate, pH 4.6, 20°C, 10 days, plate-like crystals, soaking of crystals in mother liquor with 10 mM ZnCl2, for 3 h, cryoprotection by 15% glycerol, flash freezing, and X-ray diffraction structure determination and analysis at 1.8 A resolution | Colletotrichum lindemuthianum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.41 | 0.102 | - |
2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-bromoacetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-acetamido-2-deoxy-D-glucose | pH 8.5, 30°C, continuous spectrophotometric assay | Colletotrichum lindemuthianum | |
3.5.1.41 | 0.125 | - |
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc | pH 8.5, 30°C, continuous spectrophotometric assay | Colletotrichum lindemuthianum | |
3.5.1.41 | 0.14 | - |
2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-fluoroacetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-acetamido-2-deoxy-D-glucose | pH 8.5, 30°C, continuous spectrophotometric assay | Colletotrichum lindemuthianum | |
3.5.1.41 | 0.18 | - |
2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-chloroacetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-acetamido-2-deoxy-D-glucose | pH 8.5, 30°C, continuous spectrophotometric assay | Colletotrichum lindemuthianum | |
3.5.1.41 | 0.66 | - |
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc | pH 8.5, 30°C, continuous spectrophotometric assay | Colletotrichum lindemuthianum | |
3.5.1.41 | 4.3 | - |
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc | pH 7.0, 37°C, fluorogenic labeling method assay | Colletotrichum lindemuthianum | |
3.5.1.41 | 18 | - |
GlcNAcbeta(1-4)GlcNAc | pH 8.5, 30°C, continuous spectrophotometric assay | Colletotrichum lindemuthianum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.5.1.41 | extracellular | the enzyme is secreted | Colletotrichum lindemuthianum | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.41 | Zn2+ | tightly bound to the conserved metal-binding triad consisting of His104, His108, and Asp50, structure analysis | Colletotrichum lindemuthianum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.41 | chitin + H2O | Colletotrichum lindemuthianum | - |
chitosan + acetate | - |
? | |
3.5.1.41 | chitin + H2O | Colletotrichum lindemuthianum UPS9 | - |
chitosan + acetate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.41 | Colletotrichum lindemuthianum | Q6DWK3 | - |
- |
3.5.1.41 | Colletotrichum lindemuthianum UPS9 | Q6DWK3 | - |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.5.1.41 | chitin + H2O = chitosan + acetate | ligand binding to the enzyme, computational docking analysis, catalytic mechanism via a tetrahedral oxyanion intermediate, and active site structure with a conserved metal-binding triad consisting of His104, His108, and Asp50 | Colletotrichum lindemuthianum |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.41 | 2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-bromoacetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-acetamido-2-deoxy-D-glucose + H2O | - |
Colletotrichum lindemuthianum | GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNbeta(1-4)GlcNAc + bromoacetate | - |
? | |
3.5.1.41 | 2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-bromoacetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-acetamido-2-deoxy-D-glucose + H2O | - |
Colletotrichum lindemuthianum UPS9 | GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNbeta(1-4)GlcNAc + bromoacetate | - |
? | |
3.5.1.41 | 2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-chloroacetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-acetamido-2-deoxy-D-glucose + H2O | - |
Colletotrichum lindemuthianum | GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNbeta(1-4)GlcNAc + chloroacetate | - |
? | |
3.5.1.41 | 2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-chloroacetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-acetamido-2-deoxy-D-glucose + H2O | - |
Colletotrichum lindemuthianum UPS9 | GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNbeta(1-4)GlcNAc + chloroacetate | - |
? | |
3.5.1.41 | 2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-fluoroacetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-acetamido-2-deoxy-D-glucose + H2O | - |
Colletotrichum lindemuthianum | GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNbeta(1-4)GlcNAc + fluoroacetate | - |
? | |
3.5.1.41 | chitin + H2O | - |
Colletotrichum lindemuthianum | chitosan + acetate | - |
? | |
3.5.1.41 | chitin + H2O | the enzyme is an endo-chitin de-N-acetylase | Colletotrichum lindemuthianum | chitosan + acetate | - |
? | |
3.5.1.41 | chitin + H2O | - |
Colletotrichum lindemuthianum UPS9 | chitosan + acetate | - |
? | |
3.5.1.41 | chitin + H2O | the enzyme is an endo-chitin de-N-acetylase | Colletotrichum lindemuthianum UPS9 | chitosan + acetate | - |
? | |
3.5.1.41 | GlcNAcbeta(1-4)GlcNAc + H2O | - |
Colletotrichum lindemuthianum | ? | - |
? | |
3.5.1.41 | GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | - |
Colletotrichum lindemuthianum | ? | - |
? | |
3.5.1.41 | GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | - |
Colletotrichum lindemuthianum | GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNbeta(1-4)GlcNAc + acetate | - |
? | |
3.5.1.41 | additional information | binding study of an N-acetylglucosaminyl trimer to the enzyme, computational docking | Colletotrichum lindemuthianum | ? | - |
? | |
3.5.1.41 | additional information | binding study of an N-acetylglucosaminyl trimer to the enzyme, computational docking | Colletotrichum lindemuthianum UPS9 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.1.41 | CDA | - |
Colletotrichum lindemuthianum |
3.5.1.41 | More | the enzyme belongs to the carbohydrate esterase family CE 4 | Colletotrichum lindemuthianum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.41 | 30 | - |
assay at, Escherichia coli expressed recombinant enzyme, continuous spectrophotometric assay | Colletotrichum lindemuthianum |
3.5.1.41 | 37 | - |
assay at, Pichia pastoris expressed recombinant enzyme, fluorogenic labeling method assay | Colletotrichum lindemuthianum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.41 | 1.53 | - |
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc | pH 7.0, 37°C, fluorogenic labeling method assay | Colletotrichum lindemuthianum | |
3.5.1.41 | 6 | - |
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc | pH 8.5, 30°C, continuous spectrophotometric assay | Colletotrichum lindemuthianum | |
3.5.1.41 | 7 | - |
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc | pH 8.5, 30°C, continuous spectrophotometric assay | Colletotrichum lindemuthianum | |
3.5.1.41 | 11 | - |
GlcNAcbeta(1-4)GlcNAc | pH 8.5, 30°C, continuous spectrophotometric assay | Colletotrichum lindemuthianum | |
3.5.1.41 | 31 | - |
2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-bromoacetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-acetamido-2-deoxy-D-glucose | pH 8.5, 30°C, continuous spectrophotometric assay | Colletotrichum lindemuthianum | |
3.5.1.41 | 47 | - |
2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-chloroacetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-acetamido-2-deoxy-D-glucose | pH 8.5, 30°C, continuous spectrophotometric assay | Colletotrichum lindemuthianum | |
3.5.1.41 | 68 | - |
2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-fluoroacetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-acetamido-2-deoxy-D-glucose | pH 8.5, 30°C, continuous spectrophotometric assay | Colletotrichum lindemuthianum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.41 | 7 | - |
assay at, Pichia pastoris expressed recombinant enzyme, fluorogenic labeling method assay | Colletotrichum lindemuthianum |
3.5.1.41 | 8.5 | - |
assay at, Escherichia coli expressed recombinant enzyme, continuous spectrophotometric assay | Colletotrichum lindemuthianum |