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Literature summary extracted from

  • Lehoux, I.E.; Mitra, B.
    Role of arginine 277 in (S)-mandelate dehydrogenase from Pseudomonas putida in substrate binding and transition state stabilization (2000), Biochemistry, 39, 10055-10065.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.1.99.31 R277G kcat for (S)-mandelate is 178fold lower than wild-type value, KM-value for (S)-mandelate is 160fold higher than wild-type value, KM-value for {(S)-3-phenyllactate is 1.6fold lower than wild-type value Pseudomonas putida
1.1.99.31 R277H kcat for (S)-mandelate is 1000fold lower than wild-type value, KM-value for (S)-mandelate is 608fold higher than wild-type value Pseudomonas putida
1.1.99.31 R277K kcat for (S)-mandelate is 3.7fold lower than wild-type value, KM-value for (S)-mandelate is 36.7fold higher than wild-type value Pseudomonas putida
1.1.99.31 R277L kcat for (S)-mandelate is 421fold lower fold than wild-type value, KM-value for (S)-mandelate is 392fold higher than wild-type value Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.31 0.074
-
 (S)-3-phenyllactate pH 7.5, 20°C, mutant enzyme R277G Pseudomonas putida
1.1.99.31 0.12
-
 (S)-Mandelate pH 7.5, 20°C, wild-type enzyme Pseudomonas putida
1.1.99.31 4.3
-
 (S)-3-phenyllactate pH 7.5, 20°C, wild-type enzyme Pseudomonas putida
1.1.99.31 4.4
-
 (S)-Mandelate pH 7.5, 20°C, mutant enzyme R277K Pseudomonas putida
1.1.99.31 19.2
-
 (S)-Mandelate pH 7.5, 20°C, mutant enzyme R277H Pseudomonas putida
1.1.99.31 47
-
 (S)-Mandelate pH 7.5, 20°C, mutant enzyme R277G Pseudomonas putida
1.1.99.31 73
-
 (S)-Mandelate pH 7.5, 20°C, mutant enzyme R277L Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.31 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.31 mutant enzymes R277K, R277G, R277H and R277L Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.31 (S)-3-phenyllactate + acceptor
-
 Pseudomonas putida ?
-
 ?
1.1.99.31 (S)-mandelate + acceptor
-
 Pseudomonas putida 2-oxo-2-phenylacetate + reduced acceptor
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.99.31 0.27
-
 (S)-Mandelate pH 7.5, 20°C, mutant enzyme R277L Pseudomonas putida
1.1.99.31 0.64
-
 (S)-Mandelate pH 7.5, 20°C, mutant enzyme R277G Pseudomonas putida
1.1.99.31 1.52
-
 (S)-Mandelate pH 7.5, 20°C, mutant enzyme R277H Pseudomonas putida
1.1.99.31 73
-
 (S)-Mandelate pH 7.5, 20°C, mutant enzyme R277K Pseudomonas putida
1.1.99.31 201
-
 (S)-3-phenyllactate pH 7.5, 20°C, mutant enzyme R277G Pseudomonas putida
1.1.99.31 270
-
 (S)-Mandelate pH 7.5, 20°C, wild-type enzyme Pseudomonas putida
1.1.99.31 270
-
 (S)-3-phenyllactate pH 7.5, 20°C, wild-type enzyme Pseudomonas putida