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Literature summary extracted from

  • Takenaka, S.; Murakami, S.; Kim, Y.J.; Aoki, K.
    Complete nucleotide sequence and functional analysis of the genes for 2-aminophenol metabolism from Pseudomonas sp. AP-3 (2000), Arch. Microbiol., 174, 265-272.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.74 expressed in Escherichia coli XL-1 Blue cells Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.99.5 Cu2+ slightly inhibits Pseudomonas sp.
3.5.99.5 Hg2+ slightly inhibits Pseudomonas sp.
3.5.99.5 additional information metal chelating, sulfhydryl, and surface-active agents do not affect the enzyme reaction Pseudomonas sp.
3.5.99.5 Pb2+ slightly inhibits Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.99.5 16000
-
 4 * 16000, SDS-PAGE Pseudomonas sp.
3.5.99.5 67000
-
 gel filtration Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.74 Pseudomonas sp.
-
-
-
1.13.11.74 Pseudomonas sp. Ap-3
-
-
-
3.5.99.5 Pseudomonas sp.
-
-
-
3.5.99.5 Pseudomonas sp. Ap-3
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.99.5
-
 Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
3.5.99.5 4°C, 1 month Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.74 2-aminophenol + O2
-
 Pseudomonas sp. 2-aminomuconate 6-semialdehyde
-
 ?
1.13.11.74 2-aminophenol + O2
-
 Pseudomonas sp. Ap-3 2-aminomuconate 6-semialdehyde
-
 ?
3.5.99.5 2-aminomuconate + H2O
-
 Pseudomonas sp. 4-oxalocrotonate + NH3
-
 ?
3.5.99.5 2-aminomuconate + H2O
-
 Pseudomonas sp. Ap-3 4-oxalocrotonate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.74 heterotetramer
-
 Pseudomonas sp.
3.5.99.5 homotetramer 4 * 16000, SDS-PAGE Pseudomonas sp.

Synonyms

EC Number Synonyms Comment Organism
1.13.11.74 amnBA
-
 Pseudomonas sp.
3.5.99.5 2-aminomuconate deaminase
-
 Pseudomonas sp.
3.5.99.5 amnD
-
 Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.99.5 55
-
-
 Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.99.5 7
-
-
 Pseudomonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.99.5 5.5 8
-
 Pseudomonas sp.