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Literature summary extracted from

  • Leelapon, O.; Sarath, G.; Staswick, P.E.
    A single amino acid substitution in soybean VSPalpha increases its acid phosphatase activity nearly 20-fold (2004), Planta, 219, 1071-1079.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.2 DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of GST-tagged wild-type and mutant APase in Escherichia coli strain BL21(DE3) Glycine max
3.1.3.2 DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of GST-tagged wild-type and mutant VSPalpha in Escherichia coli strain BL21(DE3) Glycine max

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.2 S106D site-directed mutagenesis of VSPalpha, the mutation increases the enzyme activity about 20fold with substrate GMP compared to the wild-type enzyme Glycine max

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.2 cAMP
-
 Glycine max
3.1.3.2 GMP
-
 Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.2 0.9
-
 GMP pH 6.0, 37°C, recombinant enzyme Glycine max
3.1.3.2 8.8
-
 4-nitrophenol phosphate pH 6.0, 37°C, recombinant enzyme Glycine max

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.2 Mg2+
-
 Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.2 27000
-
 x * 27000, recombinant enzyme, SDS-PAGE Glycine max

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.2 additional information Glycine max VSPalpha functions as temporary storage reserve and as plant acid phosphatase ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Glycine max O49855 APase
-
3.1.3.2 Glycine max P10742 VSPalpha, i.e. VSP25, fragment
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2 recombinant GST-tagged wild-type and mutant APase 3.0fold from Escherichia coli strain BL21(DE3) by ammonium sulfate precipitation and ion exchange chromatography Glycine max
3.1.3.2 recombinant GST-tagged wild-type and mutant VSPalpha from Escherichia coli strain BL21(DE3) by ammonium sulfate precipitation and ion exchange chromatography, or by glutathione affinity chromatography Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.2 root nodule APase Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.2 0.5
-
 purified recombinant enzyme, substrate phospho-L-tyrosine Glycine max
3.1.3.2 3.2
-
 purified recombinant enzyme, substrate 4-nitrophenyl phosphate Glycine max
3.1.3.2 20
-
 purified recombinant S106D mutant enzyme, substrate phospho-L-tyrosine Glycine max
3.1.3.2 22.6
-
 purified recombinant enzyme, substrate GMP Glycine max
3.1.3.2 35
-
 purified recombinant S106D mutant enzyme, substrate 4-nitrophenyl phosphate Glycine max
3.1.3.2 72.1
-
 purified recombinant GST-tagged enzyme, substrate 4-nitrophenyl phosphate Glycine max
3.1.3.2 439
-
 purified recombinant S106D mutant enzyme, substrate GMP Glycine max
3.1.3.2 845
-
 purified recombinant detagged enzyme, substrate GMP Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 4-nitrophenyl phosphate + H2O
-
 Glycine max 4-nitrophenol + phosphate
-
 ?
3.1.3.2 ADP + H2O
-
 Glycine max AMP + phosphate
-
 ?
3.1.3.2 FMN + H2O
-
 Glycine max flavin nucleoside + phosphate
-
 ?
3.1.3.2 GMP + H2O
-
 Glycine max guanosine + phosphate
-
 ?
3.1.3.2 GMP + H2O best substrate, recombinant enzyme Glycine max guanosine + phosphate
-
 ?
3.1.3.2 additional information VSPalpha functions as temporary storage reserve and as plant acid phosphatase Glycine max ?
-
 ?
3.1.3.2 additional information ADP, FMN, and triphosphate are no substrates Glycine max ?
-
 ?
3.1.3.2 additional information no activity with triphosphate Glycine max ?
-
 ?
3.1.3.2 phospho-L-tyrosine + H2O
-
 Glycine max L-tyrosine + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.2 ? x * 27000, recombinant enzyme, SDS-PAGE Glycine max

Synonyms

EC Number Synonyms Comment Organism
3.1.3.2 APase
-
 Glycine max
3.1.3.2 More VSPalpha belongs to the haloacid dehalogenase superfamily Glycine max
3.1.3.2 vegetative storage protein alpha
-
 Glycine max
3.1.3.2 VSP25
-
 Glycine max
3.1.3.2 VSPalpha
-
 Glycine max

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.2 37
-
 assay at Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 6
-
-
 Glycine max
3.1.3.2 6
-
 assay at Glycine max

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.3.2 0.015
-
 GMP pH 6.0, 37°C, recombinant GST-tagged enzyme Glycine max