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Literature summary extracted from

  • Lei, X.G.; Kim, T.
    Expression of microbial phytases in yeast systems and characterization of the recombinant enzymes (2005), Methods Biotechnol., 17, 209-224.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.8 expression in Pichia pastoris (constitutive or inducible) and Saccharomyces cerevisiae (inducible) Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Aspergillus niger P34752 3-phytase A precursor
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.8 glycoprotein recombinant enzyme expressed in yeast is highly glycosylated Aspergillus niger

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.8 partial Aspergillus niger

Synonyms

EC Number Synonyms Comment Organism
3.1.3.8 3-phytase A
-
 Aspergillus niger
3.1.3.8 PhyA phytase
-
 Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.8 55
-
 30 min, stable, enzyme expressed in Saccharomyces cerevisiae or Pichia pastoris Aspergillus niger
3.1.3.8 65
-
 30 min, enzyme expressed in Saccharomyces cerevisiae loses about 30% of initial activity, enzyme expressed in Pichia pastoris loses about 45% of initial activity Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 5
-
 enzyme expressed in Saccharomyces cerevisiae Aspergillus niger

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.8 3.5 6 pH 3.5: about 40% of maximal activity, pH 6.0: about 80% of maximal activity, pH 6.5: about 30% of maximal activity. enzyme expressed in Saccharomyces cerevisiae Aspergillus niger