EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.1.4.12 | hanging drop vapor diffusion method, crystal structures of Bc-SMase complexed to the functional metal ions Mg2+, Co2+, or Ca2+ are determined at 1.8 A, 1.8 A, and 1.4 A resolution, respectively | Bacillus cereus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.4.12 | F285A | catalytic activity of the mutant enzyme is below the detection limit, affinity of mutant enzyme to sphingomyelin liposomes are much weaker than the wild-type enzyme | Bacillus cereus |
3.1.4.12 | W284A | catalytic activity of the mutant enzyme is below the detection limit, affinity of mutant enzyme to sphingomyelin liposomes are much weaker than the wild-type enzyme | Bacillus cereus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.4.12 | Ca2+ | Km: 0.0191 mM, activation of sphingomyelinase activity in the order of decreasing efficiency Co2+, Mn2+, Mg2+, Ca2+, Sr2+ | Bacillus cereus | |
3.1.4.12 | Co2+ | Km: 0.142 mM, activation of sphingomyelinase activity in the order of decreasing efficiency Co2+, Mn2+, Mg2+, Ca2+, Sr2+. There are three distinct metal ion-binding sites in a long horizontal cleft across the bc-SMase molecule | Bacillus cereus | |
3.1.4.12 | Mg2+ | Km: 0.0161 mM, activation of sphingomyelinase activity in the order of decreasing efficiency Co2+, Mn2+, Mg2+, Ca2+, Sr2+ | Bacillus cereus | |
3.1.4.12 | Mn2+ | Km: 0.0149 mM, activation of sphingomyelinase activity in the order of decreasing efficiency Co2+, Mn2+, Mg2+, Ca2+, Sr2+ | Bacillus cereus | |
3.1.4.12 | Sr2+ | Km: 0.018 mM, activation of sphingomyelinase activity in the order of decreasing efficiency Co2+, Mn2+, Mg2+, Ca2+, Sr2+ | Bacillus cereus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.4.12 | Bacillus cereus | P11889 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.4.12 | sphingomyelin + H2O | - |
Bacillus cereus | N-acylsphingosine + choline phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.4.12 | Bc-SMase | - |
Bacillus cereus |
3.1.4.12 | neutral sphingomyelinase | - |
Bacillus cereus |