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Literature summary extracted from

  • Alexa, A.; Bozóky, Z.; Farkas, A.; Tompa, P.; Friedrich, P.
    Contribution of distinct structural elements to activation of calpain by Ca2+ ions (2004), J. Biol. Chem., 279, 20118-20126.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.B37 Ca2+
-
 Drosophila melanogaster

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.B37 expression in Escherichia coli Drosophila melanogaster

Protein Variants

EC Number Protein Variants Comment Organism
3.4.22.B37 BIII-D613N/D614N retains 30% of the wild-type Ca2+-binding capacity Drosophila melanogaster
3.4.22.B37 BIII-D617N/D618 retains 60% of the wild-type Ca2+-binding capacity Drosophila melanogaster
3.4.22.B37 BIII-E610A/D611A/P612A, delta613-620 deletion mutant unable to bind Ca2+ Drosophila melanogaster
3.4.22.B37 BIII-E610Q/D611N/D613N/D614N retains 16.2% of the wild-type Ca2+-binding capacity Drosophila melanogaster
3.4.22.B37 BIII-E615Q/D616N/D617N/D618N retains 36% of the wild-type Ca2+-binding capacity Drosophila melanogaster
3.4.22.B37 BIII-P612A, delta613-620 deletion mutant retains one-third of the original Ca2+- binding capacity Drosophila melanogaster
3.4.22.B37 D613N/D614N enhances specific activity to 114% of the wild-type activity Drosophila melanogaster
3.4.22.B37 D617N/D618N reduces specific activity to 66% of the wild-type activity Drosophila melanogaster

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.22.B37 Ca2+ activates calpain Drosophila melanogaster

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.B37 Drosophila melanogaster Q9VT65
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.B37
-
 Drosophila melanogaster

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.4.22.B37 removal of urea by stepwise dialysis in buffer A (50 mM Tris, pH 7.5, 150 mM NaCl) containing 1 M NaCl and 8-4-2-1-0.5-0 M urea, at least for 8 hours in each case Drosophila melanogaster

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.B37 N-succinyl-Leu-Tyr-7-amido-4-methyl-coumarin + H2O pH 7.5 Drosophila melanogaster ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.22.B37 calpain
-
 Drosophila melanogaster
3.4.22.B37 calpain B
-
 Drosophila melanogaster