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Literature summary extracted from

  • Wang, Q.; He, P.; Lu, D.; Shen, A.; Jiang, N.
    Purification, characterization, cloning and expression of pyruvate decarboxylase from Torulopsis glabrata IFO005 (2004), J. Biochem., 136, 447-455.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.1 pyruvate
-
 [Candida] glabrata

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.1 Ag+ complete inhibition at 20 mM [Candida] glabrata
4.1.1.1 Cu+ complete inhibition at 20 mM [Candida] glabrata
4.1.1.1 glyoxylate complete inhibition [Candida] glabrata
4.1.1.1 thiamine diphosphate PDC I, complete inhibition at 75 mM, PDC II, complete inhibition at 100 mM [Candida] glabrata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.1 0.8
-
 pyruvate isoform PDC I, pH 6.0, 30°C [Candida] glabrata
4.1.1.1 0.9
-
 pyruvate isoform PDC II, pH 6.0, 30°C [Candida] glabrata

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.1 Mg2+ required [Candida] glabrata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.1 58700
-
 2 * 58700, SDS-PAGE, 2 * 62000, calculated, both isoform PDC I and PDC II [Candida] glabrata
4.1.1.1 62000
-
 2 * 58700, SDS-PAGE, 2 * 62000, calculated, both isoform PDC I and PDC II [Candida] glabrata
4.1.1.1 120000
-
 PAGE [Candida] glabrata
4.1.1.1 150000
-
 gel filtration [Candida] glabrata

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.1 [Candida] glabrata Q6FJA3 strain IFO005, isoforms PDC I and PDC II
-
4.1.1.1 [Candida] glabrata CBS 138 Q6FJA3 strain IFO005, isoforms PDC I and PDC II
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.1
-
 [Candida] glabrata

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.1 21.7
-
 isoform PDC I, pH 6.0, 30°C [Candida] glabrata
4.1.1.1 40
-
 isoform PDC II, pH 6.0, 30°C [Candida] glabrata

Storage Stability

EC Number Storage Stability Organism
4.1.1.1 -20°C, lyophilized preparation, stable for at least 3 months [Candida] glabrata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.1 pyruvate
-
 [Candida] glabrata acetaldehyde + CO2
-
 ?
4.1.1.1 pyruvate
-
 [Candida] glabrata CBS 138 acetaldehyde + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.1 dimer 2 * 58700, SDS-PAGE, 2 * 62000, calculated, both isoform PDC I and PDC II [Candida] glabrata

Synonyms

EC Number Synonyms Comment Organism
4.1.1.1 PDC I
-
 [Candida] glabrata
4.1.1.1 PDC II
-
 [Candida] glabrata

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.1 40
-
 1 h, 73.2% residual activity, isoform PDC, 93.2% residual activity, isoform PDC II [Candida] glabrata
4.1.1.1 50
-
 1 h, 23.9% residual activity, isoform PDC, 43.1% residual activity, isoform PDC II [Candida] glabrata
4.1.1.1 60
-
 1 h, 3% residual activity, isoform PDC, 2.7% residual activity, isoform PDC II [Candida] glabrata

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.1 43.6
-
 pyruvate isoform PDC I, pH 6.0, 30°C [Candida] glabrata
4.1.1.1 77
-
 pyruvate isoform PDC II, pH 6.0, 30°C [Candida] glabrata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.1 6 6.5
-
 [Candida] glabrata

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.1.1 6.5 7.5
-
 [Candida] glabrata

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.1 thiamine diphosphate
-
 [Candida] glabrata