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Literature summary extracted from

  • Kimura, T.; Vassilev, V.P.; Shen, G.J.; Wong, C.H.
    Enzymic synthesis of beta-hydroxy-alpha-amino acids based on recombinant D- and L-threonine aldolases (1997), J. Am. Chem. Soc., 119, 11734-11742.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.42 overexpression in Escherichia coli Xanthomonas oryzae

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.42 acetonitrile inactivates Xanthomonas oryzae
4.1.2.42 dimethylformamide inactivates Xanthomonas oryzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.42 0.94
-
 D-threonine
-
 Xanthomonas oryzae
4.1.2.42 0.99
-
 D-allo-threonine
-
 Xanthomonas oryzae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.2.42 Mg2+ required Xanthomonas oryzae

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.42 Xanthomonas oryzae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.42 recombinant Xanthomonas oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.42 D-allo-threonine
-
 Xanthomonas oryzae glycine + acetaldehyde
-
 r
4.1.2.42 D-threonine
-
 Xanthomonas oryzae glycine + acetaldehyde
-
 r
4.1.2.42 glycine + (4R)-2,2-dimethyl-1,3-dioxolane-4-carbaldehyde
-
 Xanthomonas oryzae (2R,3R)-2-amino-3-[(4R)-2,2-dimethyl-1,3-dioxolan-4-yl]-3-hydroxypropanoic acid + (2R,3S)-2-amino-3-[(4R)-2,2-dimethyl-1,3-dioxolan-4-yl]-3-hydroxypropanoic acid
-
 r
4.1.2.42 glycine + (4S)-2,2-dimethyl-1,3-dioxolane-4-carbaldehyde
-
 Xanthomonas oryzae (2R,3R)-2-amino-3-[(4S)-2,2-dimethyl-1,3-dioxolan-4-yl]-3-hydroxypropanoic acid + (2R,3S)-2-amino-3-[(4S)-2,2-dimethyl-1,3-dioxolan-4-yl]-3-hydroxypropanoic acid
-
 ?
4.1.2.42 glycine + 2-methylpropanal
-
 Xanthomonas oryzae (2R,3S)-2-amino-3-hydroxy-4-methylpentanoic acid + (2R,3R)-2-amino-3-hydroxy-4-methylpentanoic acid
-
 r
4.1.2.42 glycine + 2-nitrobenzaldehyde
-
 Xanthomonas oryzae (2R,3S)-2-amino-3-hydroxy-3-(2-nitrophenyl)propanoic acid + (2R,3R)-2-amino-3-hydroxy-3-(2-nitrophenyl)propanoic acid
-
 r
4.1.2.42 glycine + 3-hydroxybenzaldehyde
-
 Xanthomonas oryzae (2R,3S)-2-amino-3-hydroxy-3-(3-hydroxyphenyl)propanoic acid + (2R,3R)-2-amino-3-hydroxy-3-(3-nitrophenyl)propanoic acid
-
 r
4.1.2.42 glycine + 4-fluoro-3-nitrobenzaldehyde
-
 Xanthomonas oryzae (2R,3S)-2-amino-3-(4-fluoro-3-nitrophenyl)-3-hydroxypropanoic acid + (2R,3R)-2-amino-3-(4-fluoro-3-nitrophenyl)-3-hydroxypropanoic acid
-
 r
4.1.2.42 glycine + 4-methylbenzaldehyde
-
 Xanthomonas oryzae (2R,3S)-2-amino-3-hydroxy-3-(4-methylphenyl)propanoic acid + (2R,3R)-2-amino-3-hydroxy-3-(4-nitrophenyl)propanoic acid
-
 r
4.1.2.42 glycine + 4-nitrobenzaldehyde
-
 Xanthomonas oryzae (2R,3S)-2-amino-3-hydroxy-3-(4-nitrophenyl)propanoic acid + (2R,3R)-2-amino-3-hydroxy-3-(4-nitrophenyl)propanoic acid
-
 r
4.1.2.42 glycine + acetaldehyde
-
 Xanthomonas oryzae D-threonine + D-allo-threonine
-
 r
4.1.2.42 glycine + benzaldehyde
-
 Xanthomonas oryzae (2R,3S)-2-amino-3-hydroxy-3-phenylpropanoic acid + (2R,3R)-2-amino-3-hydroxy-3-phenylpropanoic acid
-
 r
4.1.2.42 glycine + butyraldehyde
-
 Xanthomonas oryzae (2R,3S)-2-amino-3-hydroxyhexanoic acid + (2R,3R)-2-amino-3-hydroxyhexanoic acid
-
 r
4.1.2.42 glycine + hexanaldehyde
-
 Xanthomonas oryzae (2R,3R)-2-amino-3-hydroxyoctanoic acid + (2R,3S)-2-amino-3-hydroxyoctanoic acid
-
 r
4.1.2.42 glycine + octanaldehyde
-
 Xanthomonas oryzae (2R,3R)-2-amino-3-hydroxydecanoic acid + (2R,3S)-2-amino-3-hydroxydecanoic acid
-
 r
4.1.2.42 glycine + phenylacetaldehyde
-
 Xanthomonas oryzae (2R,3S)-2-amino-3-hydroxy-4-phenylbutanoic acid + (2R,3R)-2-amino-3-hydroxy-4-phenylbutanoic acid
-
 r
4.1.2.42 additional information no activity with but-2-enal, 4-hydroxybenzaldehyde Xanthomonas oryzae ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.2.42 DTA
-
 Xanthomonas oryzae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.2.42 37
-
-
 Xanthomonas oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.42 7.5
-
 aldol addition Xanthomonas oryzae
4.1.2.42 8
-
 cleavage reaction Xanthomonas oryzae

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.2.42 pyridoxal 5'-phosphate required to activate glycine Xanthomonas oryzae