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Literature summary extracted from

  • Oda, K.
    Sedolisin-B (2004), Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press, 2, 1888-1889.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.101 expression in Escherichia coli Xanthomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.101 isovaleryl-tyrosyl-leucyl-tyrosinal tyrostatin Xanthomonas sp.
3.4.21.101 additional information no inhibition by pepstatin, 2,3-diamino naphthalene and 1,2-Epoxy-3-(p-nitrophenoxy)propane Xanthomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.101 0.0036
-
 1-(4-methoxyphenyl)-2-methyl-3-nitro-1-H-indol-6-ol pH3.5, 37°C Xanthomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.101 41000
-
 2 * 41000, SDS-PAGE Xanthomonas sp.
3.4.21.101 84000
-
 recombinant protein from Escherichia coli Xanthomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.101 Xanthomonas sp. Q60106 T-22
-
3.4.21.101 Xanthomonas sp. T-22 Q60106 T-22
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.101 Acid-denatured hemoglobin + H2O
-
 Xanthomonas sp. ?
-
 ?
3.4.21.101 Acid-denatured hemoglobin + H2O
-
 Xanthomonas sp. T-22 ?
-
 ?
3.4.21.101 casein + H2O
-
 Xanthomonas sp. ?
-
 ?
3.4.21.101 casein + H2O
-
 Xanthomonas sp. T-22 ?
-
 ?
3.4.21.101 Lys-Pro-Ala-Asp-Phe-(4-nitro)Phe-Arg-Leu + H2O pH 3.5, 37°C Xanthomonas sp. Lys-Pro-Ala-Asp-Phe + (4-nitro)Phe-Arg-Leu
-
 ?
3.4.21.101 Lys-Pro-Ala-Asp-Phe-(4-nitro)Phe-Arg-Leu + H2O pH 3.5, 37°C Xanthomonas sp. T-22 Lys-Pro-Ala-Asp-Phe + (4-nitro)Phe-Arg-Leu
-
 ?
3.4.21.101 [protein]-3-O-(alpha-D-GlcNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine + H2O
-
 Xanthomonas sp. ?
-
 ?
3.4.21.101 [protein]-3-O-(alpha-D-GlcNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine + H2O
-
 Xanthomonas sp. T-22 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.101 dimer 2 * 41000, SDS-PAGE Xanthomonas sp.

Synonyms

EC Number Synonyms Comment Organism
3.4.21.101 sedolisin-B
-
 Xanthomonas sp.
3.4.21.101 Xanthomonas serine-carboxyl peptidase
-
 Xanthomonas sp.
3.4.21.101 XSCO
-
 Xanthomonas sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.101 52.2
-
 1-(4-methoxyphenyl)-2-methyl-3-nitro-1-H-indol-6-ol pH3.5, 37°C Xanthomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.101 2.7
-
 for casein digestion Xanthomonas sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.21.101 0.0000021
-
 isovaleryl-tyrosyl-leucyl-tyrosinal
-
 Xanthomonas sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.21.101 Xanthomonas sp.
-
-
 4