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Literature summary extracted from

  • Zinin, N.V.; Serkina, A.V.; Gelfand, M.S.; Shevelev, A.B.; Sineoky, S.P.
    Gene cloning, expression and characterization of novel phytase from Obesumbacterium proteus (2004), FEMS Microbiol. Lett., 236, 283-290.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.8 expression in Escherichia coli Obesumbacterium proteus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.8 0.34
-
 myo-inositol hexakisphosphate
-
 Obesumbacterium proteus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.8 45000
-
 x * 45000, SDS-PAGE Obesumbacterium proteus

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Obesumbacterium proteus Q6U677
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.8 310
-
-
 Obesumbacterium proteus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 D-fructose 1-phosphate + H2O poor substrate Obesumbacterium proteus D-fructose + phosphate
-
 ?
3.1.3.8 D-glucose 1-phosphate + H2O poor substrate Obesumbacterium proteus D-glucose + phosphate
-
 ?
3.1.3.8 D-glucose 6-phosphate + H2O poor substrate Obesumbacterium proteus D-glucose + phosphate
-
 ?
3.1.3.8 D-ribose 5-phosphate + H2O poor substrate Obesumbacterium proteus D-ribose + phosphate
-
 ?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
 Obesumbacterium proteus ? + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.8 ? x * 45000, SDS-PAGE Obesumbacterium proteus

Synonyms

EC Number Synonyms Comment Organism
3.1.3.8 PhyA
-
 Obesumbacterium proteus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.8 40 50
-
 Obesumbacterium proteus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.3.8 30 65 30°C: about 55% of maximal activity, 65°C: about 40% of maximal activity, substrate: myo-inositol hexakisphosphate Obesumbacterium proteus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.8 50
-
 30 min, enzyme is highly resistant but loses its activity very rapidly under further increase of the temperature Obesumbacterium proteus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 3
-
 substrate: D-ribose 5-phosphate Obesumbacterium proteus
3.1.3.8 3.5
-
 substrate: D-glucose 1-phosphate, D-glucose 6-phosphate and D-fructose 1-phosphate Obesumbacterium proteus
3.1.3.8 4.9
-
 and a local pH-optimum at pH 3.4, substrate: myo-inositol hexakisphosphate Obesumbacterium proteus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.8 2 6 pH 2.0: about 45% of maximal activiry, pH 6.0: about 50% of maximal activity, substrate: myo-inositol hexakisphosphate Obesumbacterium proteus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.8 1.5 8 37°C, 0.2 M buffer solution, no significant change in activity after 24 h Obesumbacterium proteus