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Literature summary extracted from

  • Apichaisataienchote, B.; Altenbuchner, J.; Buchenauer, H.
    Isolation and identification of Streptomyces fradiae SU-1 from Thailand and protoplast transformation with the chitinase B gene from Nocardiopsis prasina OPC-131 (2005), Curr. Microbiol., 51, 116-121.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.14 gene chiB337, functional expression in Streptomyces fradiae strain SU-1 using the Escherichia coli-Streptomyces shuttle vector pFIS318 for transformation of protoplasts, secretion of chitinases by the recombinant strain, subcloning in Escherichia coli strain JM109 Nocardiopsis prasina

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.14 extracellular two extracellular isozymes are secreted to the culture medium Streptomyces fradiae
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 26000
-
 x * 26000, isozyme 1, SDS-PAGE, x * 43000, isozyme 2, SDS-PAGE Streptomyces fradiae
3.2.1.14 31500
-
 x * 31500, recombinant enzyme, SDS-PAGE Nocardiopsis prasina
3.2.1.14 43000
-
 x * 26000, isozyme 1, SDS-PAGE, x * 43000, isozyme 2, SDS-PAGE Streptomyces fradiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.14 additional information Nocardiopsis prasina the enzyme shows antifungal activity against Fusarium moniliforme, overview ?
-
 ?
3.2.1.14 additional information Nocardiopsis prasina OPC-131 the enzyme shows antifungal activity against Fusarium moniliforme, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Nocardiopsis prasina
-
 gene chiB337
-
3.2.1.14 Nocardiopsis prasina OPC-131
-
 gene chiB337
-
3.2.1.14 Streptomyces fradiae
-
 two extracellular isozymes
-
3.2.1.14 Streptomyces fradiae SU-1
-
 two extracellular isozymes
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.14 additional information
-
-
 Streptomyces fradiae
3.2.1.14 additional information
-
-
 Nocardiopsis prasina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside + H2O
-
 Streptomyces fradiae ?
-
 ?
3.2.1.14 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside + H2O
-
 Nocardiopsis prasina ?
-
 ?
3.2.1.14 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside + H2O
-
 Streptomyces fradiae SU-1 ?
-
 ?
3.2.1.14 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside + H2O
-
 Nocardiopsis prasina OPC-131 ?
-
 ?
3.2.1.14 additional information the enzyme shows antifungal activity against Fusarium moniliforme, overview Nocardiopsis prasina ?
-
 ?
3.2.1.14 additional information the enzyme shows antifungal activity against Fusarium moniliforme, overview Nocardiopsis prasina OPC-131 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 ? x * 26000, isozyme 1, SDS-PAGE, x * 43000, isozyme 2, SDS-PAGE Streptomyces fradiae
3.2.1.14 ? x * 31500, recombinant enzyme, SDS-PAGE Nocardiopsis prasina

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 chitinase B
-
 Nocardiopsis prasina