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Literature summary extracted from

  • Fahmy, A.S.; Abdel-Gany, S.S.; Mohamed, T.M.; Mohamed, S.A.
    Esterase and lipase in camel tick Hyalomma dromedarii (Acari: Ixodidae) during embryogenesis (2004), Comp. Biochem. Physiol. B, 137B, 159-168.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 4-hydroxymercuribenzoate competitive inhibition of LIII, 60% inhibition at 5 mM Hyalomma dromedarii
3.1.1.3 Ca2+ 50% inhibition at 1 mM Hyalomma dromedarii
3.1.1.3 DTNB competitive inhibition of LIII, 30% inhibition at 5 mM Hyalomma dromedarii
3.1.1.3 Fe3+ 68% inhibition at 1 mM Hyalomma dromedarii
3.1.1.3 Hg2+ 75% inhibition at 1 mM Hyalomma dromedarii
3.1.1.3 Li+ 56% inhibition at 1 mM Hyalomma dromedarii
3.1.1.3 Mn2+ 81% inhibition at 1 mM Hyalomma dromedarii
3.1.1.3 additional information no inhibition by 2-mercaptoethanol, PMSF, and iodoacetamide Hyalomma dromedarii
3.1.1.3 NEM 10% inhibition at 5 mM Hyalomma dromedarii
3.1.1.3 Ni2+ 86% inhibition at 1 mM Hyalomma dromedarii
3.1.1.3 Zn2+ 98% inhibition at 1 mM Hyalomma dromedarii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.3 additional information
-
 additional information
-
 Hyalomma dromedarii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.3 additional information the enzyme is not affected by Mg2+ at 1 mM Hyalomma dromedarii

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Hyalomma dromedarii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 native enzyme partially by ion exchange chromatography, separation from esterases Hyalomma dromedarii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.3 embryo the enzyme activity changes during embryogenesis Hyalomma dromedarii
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 0.063
-
 partially purified enzyme Hyalomma dromedarii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 triolein + H2O olive oil Hyalomma dromedarii diolein + oleate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 lipase
-
 Hyalomma dromedarii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 40
-
-
 Hyalomma dromedarii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 70
-
 loss of 53% activity after 15 min Hyalomma dromedarii
3.1.1.3 90
-
 15 min, inactivation Hyalomma dromedarii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 8
-
-
 Hyalomma dromedarii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.1.3 0.2
-
 4-hydroxymercuribenzoate pH 8.0, 37°C Hyalomma dromedarii
3.1.1.3 0.25
-
 DTNB pH 8.0, 37°C Hyalomma dromedarii