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Literature summary extracted from

  • Jiang, Y.; Ye, J.; Wu, H.; Zhang, H.
    Cloning and expression of the polyhydroxyalkanoate depolymerase gene from Pseudomonas putida, and characterization of the gene product (2004), Biotechnol. Lett., 26, 1585-1588.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.76 gene phaZ, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) in inclusion bodies Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.76 31283
-
 x * 31283, sequence calculation Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.76 Pseudomonas putida Q6PLI2 gene phaZ
-
3.1.1.76 Pseudomonas putida H14 Q6PLI2 gene phaZ
-

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.1.1.76 denaturing of the enzyme by Triton buffer, refolding of the enzyme from inclusion bodies after recombinant expression in Escherichia coli in Tris-HCl, pH 8.0, containing GSSG and GSH at 25°C Pseudomonas putida

Subunits

EC Number Subunits Comment Organism
3.1.1.76 ? x * 31283, sequence calculation Pseudomonas putida
3.1.1.76 More the primary sequence contains a conserved lipase box-lke sequence G-X-S102-X-G Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
3.1.1.76 PHA depolymerase
-
 Pseudomonas putida
3.1.1.76 polyhydroxyalkanoate depolymerase
-
 Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.76 40
-
-
 Pseudomonas putida

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.76 10 40 maximal activity at 40°C, 50% activity of maximal activity at 10°C Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.76 8
-
-
 Pseudomonas putida