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Literature summary extracted from

  • Johnson, W.H., Jr.; Wang, S.C.; Stanley, T.M.; Czerwinski, R.M.; Almrud, J.J.; Poelarends, G.J.; Murzin, A.G.; Whitman, C.P.
    4-Oxalocrotonate tautomerase, its homologue YwhB, and active vinylpyruvate hydratase: synthesis and evaluation of 2-fluoro substrate analogues (2004), Biochemistry, 43, 10490-10501.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.77 E109Q 7fold increase in Km-value, 2270fold decrease in kcat, no influence of mutation to vinylpyruvate hydratase activity Pseudomonas putida
4.1.1.77 E111Q Km-value comparable to wild-type, 3400fold decrease in kcat no influence of mutation to vinylpyruvate hydratase activity Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.77 (2E)-2-fluorohex-2-en-4-ynoic acid
-
 Pseudomonas putida
4.1.1.77 (2E)-2-fluoropent-2-en-4-ynoic acid
-
 Pseudomonas putida
4.1.1.77 (2E)-2-fluoropenta-2,4-dienoic acid
-
 Pseudomonas putida
4.1.1.77 (2E,4E)-2-fluorohexa-2,4-dienedioic acid
-
 Pseudomonas putida
4.1.1.77 (2E,4E)-2-fluorohexa-2,4-dienoic acid
-
 Pseudomonas putida
4.1.1.77 (2Z)-2-fluorohex-2-en-4-ynedioic acid
-
 Pseudomonas putida
4.1.1.77 (2Z)-2-fluoropent-2-en-4-ynoic acid
-
 Pseudomonas putida
4.1.1.77 (2Z)-2-fluoropenta-2,4-dienoic acid
-
 Pseudomonas putida
4.1.1.77 (2Z,4E)-2-fluorohexa-2,4-dienoic acid
-
 Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.77 0.006
-
 4-oxalocrotonate mutant E111Q, in complex with vinylpuruvate hydratase Pseudomonas putida
4.1.1.77 0.0066
-
 4-oxalocrotonate wild-type, in complex with vinylpuruvate hydratase Pseudomonas putida
4.1.1.77 0.047
-
 4-oxalocrotonate mutant E109Q, in complex with vinylpuruvate hydratase Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.77 Pseudomonas putida
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.77 4-Oxalocrotonate
-
 Pseudomonas putida 2-Oxopent-4-enoate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.77 More enzyme forms a complex with vinylpyruvate hydratase, which is only active when stabilized in this complex Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.77 0.02
-
 4-oxalocrotonate mutant E111Q, in complex with vinylpuruvate hydratase Pseudomonas putida
4.1.1.77 0.03
-
 4-oxalocrotonate mutant E109Q, in complex with vinylpuruvate hydratase Pseudomonas putida
4.1.1.77 68
-
 4-oxalocrotonate wild-type, in complex with vinylpuruvate hydratase Pseudomonas putida

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.1.77 0.00011
-
 (2E)-2-fluoropenta-2,4-dienoic acid pH 7.3, 22°C Pseudomonas putida
4.1.1.77 0.0003
-
 (2Z)-2-fluorohex-2-en-4-ynedioic acid pH 7.3, 22°C Pseudomonas putida
4.1.1.77 0.00034
-
 (2Z)-2-fluoropenta-2,4-dienoic acid pH 7.3, 22°C Pseudomonas putida
4.1.1.77 0.0022
-
 (2E,4E)-2-fluorohexa-2,4-dienoic acid pH 7.3, 22°C Pseudomonas putida
4.1.1.77 0.0068
-
 (2E)-2-fluorohex-2-en-4-ynoic acid pH 7.3, 22°C Pseudomonas putida
4.1.1.77 0.0085
-
 (2Z,4E)-2-fluorohexa-2,4-dienoic acid pH 7.3, 22°C Pseudomonas putida
4.1.1.77 0.014
-
 (2E)-2-fluoropent-2-en-4-ynoic acid pH 7.3, 22°C Pseudomonas putida
4.1.1.77 0.045
-
 (2E,4E)-2-fluorohexa-2,4-dienedioic acid pH 7.3, 22°C Pseudomonas putida
4.1.1.77 0.05
-
 (2Z)-2-fluoropent-2-en-4-ynoic acid pH 7.3, 22°C Pseudomonas putida