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Literature summary extracted from
- Functional reconstitution of a crenarchaeal splicing endonuclease in vitro (2005), Biochem. Biophys. Res. Commun., 334, 1254-1259.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.6.1.16 | two distinct genes endA, ORFs ST0358 and STS047, DNA and amino acid sequence determination and analysis of two ORFs encoding the enzyme, one ORF lacks the putative catalytic amino acid residue, individual expression in Escherichia coli reveals that both are components of an enzyme complex | Sulfurisphaera tokodaii |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.6.1.16 | Mg2+ | - |
Sulfurisphaera tokodaii |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.6.1.16 | Sulfurisphaera tokodaii | - |
heteromeric enzyme complex composed of two components | - |
| 4.6.1.16 | Sulfurisphaera tokodaii 7 | - |
heteromeric enzyme complex composed of two components | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.6.1.16 | additional information | the heteromeric enzyme complex is composed of two components which are both required for catalytic activity, analysis of reaction products with synthetic tRNA substrate, overview | Sulfurisphaera tokodaii | ? | - |
? | |
| 4.6.1.16 | additional information | the heteromeric enzyme complex is composed of two components which are both required for catalytic activity, analysis of reaction products with synthetic tRNA substrate, overview | Sulfurisphaera tokodaii 7 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.6.1.16 | dimer | heteromeric enzyme complex, both enzyme components are required for catalytic activity | Sulfurisphaera tokodaii |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.6.1.16 | splicing endonuclease | - |
Sulfurisphaera tokodaii |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.6.1.16 | 70 | - |
assay at | Sulfurisphaera tokodaii |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.6.1.16 | 8.5 | - |
assay at | Sulfurisphaera tokodaii |
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Release 2023.1 (January 2023)
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