EC Number | Application | Comment | Organism |
---|---|---|---|
1.2.4.1 | biotechnology | active expression of enzyme from non-halophilic Zymomonas mobilis in the haloarchaeon Haloferax volcanii with no difference in the secondary structure. Post-transcriptional mechanisms in the stationary phase appear to limit the amount of recombinant protein expressed | Zymomonas mobilis |
EC Number | General Stability | Organism |
---|---|---|
4.1.1.1 | retains about 50% of activity in buffer supplemented with 2 M NaCl or KCl, retain 20% of activity in 4 M salt buffer | Zymomonas mobilis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.2.4.1 | KCl | at 2 M, 50% residual activity of enzyme expressed in Escherichia coli | Zymomonas mobilis | |
1.2.4.1 | NaCl | at 2 M, 50% residual activity of enzyme expressed in Escherichia coli | Zymomonas mobilis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.2.4.1 | 60000 | - |
4 * 60000, SDS-PAGE | Zymomonas mobilis |
1.2.4.1 | 240000 | - |
gel filtration, recombinant protein from Escherichia coli and from Haloferax volcanii | Zymomonas mobilis |
4.1.1.1 | 60000 | - |
4 * 60000, SDS-PAGE | Zymomonas mobilis |
4.1.1.1 | 240000 | - |
gel filtration | Zymomonas mobilis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.4.1 | Zymomonas mobilis | - |
recombinant expression in Haloferax volcanii | - |
4.1.1.1 | Zymomonas mobilis | - |
expression in Haloferax volcanii, growth-phase dependent decrease in amount of enzyme protein | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.4.1 | recombinant enzyme both expressed in Escherichia coli and Haloferax volcanii | Zymomonas mobilis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.2.4.1 | 55 | - |
recombinant enzyme from Haloferax volcanii | Zymomonas mobilis |
1.2.4.1 | 91 | - |
recombinant enzyme from Escherichia coli | Zymomonas mobilis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.4.1 | pyruvate + CoA + NAD+ | - |
Zymomonas mobilis | acetyl-CoA + CO2 + NADH | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.2.4.1 | tetramer | 4 * 60000, SDS-PAGE | Zymomonas mobilis |
4.1.1.1 | tetramer | 4 * 60000, SDS-PAGE | Zymomonas mobilis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.4.1 | NAD+ | - |
Zymomonas mobilis |