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Literature summary extracted from

  • Berensmeier, S.; Singh, S.A.; Meens, J.; Buchholz, K.
    Cloning of the pelA gene from Bacillus licheniformis 14A and biochemical characterization of recombinant, thermostable, high-alkaline pectate lyase (2004), Appl. Microbiol. Biotechnol., 64, 560-567.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.22 expression in Escherichia coli as a recombinant fusion protein containing a C-terminal His-tag Bacillus licheniformis

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.22 polygalacturonic acid recombinant fusion protein containing a C-terminal His-tag is inhibited by excess substrate Bacillus licheniformis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.22 additional information
-
 additional information Km-value for polygalacturonic acid is 0.56 g/l, recombinant fusion protein containing a C-terminal His-tag Bacillus licheniformis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.22 33451
-
 x * 33451, calculated from sequence Bacillus licheniformis
4.2.2.22 36000
-
 x * 36000, SDS-PAGE Bacillus licheniformis

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.22 Bacillus licheniformis Q8GCB2
-
-
4.2.2.22 Bacillus licheniformis 14A Q8GCB2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.22 recombinant Bacillus licheniformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.22 polygalacturonic acid
-
 Bacillus licheniformis unsaturated trigalacturonate main product ?
4.2.2.22 polygalacturonic acid
-
 Bacillus licheniformis 14A unsaturated trigalacturonate main product ?

Subunits

EC Number Subunits Comment Organism
4.2.2.22 ? x * 36000, SDS-PAGE Bacillus licheniformis
4.2.2.22 ? x * 33451, calculated from sequence Bacillus licheniformis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.22 65
-
 recombinant fusion protein containing a C-terminal His-tag Bacillus licheniformis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.2.22 additional information
-
 additional information Ki-value for polygalacturonic acid is 1.08 g/l Bacillus licheniformis