EC Number | Cloned (Comment) | Organism |
---|---|---|
5.3.1.12 | overexpression in Escherichia coli BL21(DE3)pLysS | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
5.3.1.12 | EDTA | 0.25 mM, 50% inhibition | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.1.12 | 0.29 | - |
D-glucuronate | in absence of added metal or chelator | Escherichia coli | |
5.3.1.12 | 0.31 | - |
D-glucuronate | in presence of 0.01 mM Zn2+ | Escherichia coli | |
5.3.1.12 | 0.41 | - |
D-glucuronate | in presence of 0.01 mM Co2+ | Escherichia coli | |
5.3.1.12 | 1.2 | - |
D-glucuronate | in presence of 0.1 mM Mn2+ | Escherichia coli |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
5.3.1.12 | Co2+ | stimulation is more potent at lower concentrations than stimulation by Mn2+ | Escherichia coli | |
5.3.1.12 | Mn2+ | maximal stimulation is higher than stimulatipn by Zn2+ or Co2+, stimulation at low concentrations is lower than stimulation by Zn2+ and Co2+ | Escherichia coli | |
5.3.1.12 | Zn2+ | stimulation is more potent at lower concentrations than stimulation by Mn2+. Vmax is about 3times higher in presence of 0.1 mM Mn2+ than in presence of 0.01 mM Zn2+ or Co2+ | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.3.1.12 | Escherichia coli | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.3.1.12 | - |
Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.3.1.12 | D-Glucuronate | - |
Escherichia coli | D-Fructuronate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.3.1.12 | Uronate isomerase | - |
Escherichia coli |