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Literature summary extracted from

  • Yuan, J.; Sayegh, J.; Mendez, J.; Sward, L.; Sanchez, N.; Sanchez, S.; Waldrop, G.; Grover, S.
    The regulatory role of residues 226-232 in phosphoenolpyruvate carboxylase from maize (2006), Photosynth. Res., 88, 73-81.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.31 glucose 6-phosphate
-
 Zea mays
4.1.1.31 glycine
-
 Zea mays

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.31 expression in Escherichia coli BL21(DE3)pLysS Zea mays

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.31 D228N reduced apparent affinity for the activator glycine Zea mays
4.1.1.31 E229A maximal activation caused by glycine is greatly reduced, significantly lowered sensitivity to the inhibitors malate and aspartate. K(0.5) for phosphoenolpyruvate is lower than wild-type value Zea mays
4.1.1.31 R226Q maximal activation caused by glycine is greatly reduced, significantly lowered sensitivity to the inhibitors malate and aspartate. K(0.5) for phosphoenolpyruvate is significantly higher than that of wild-type enzyme Zea mays
4.1.1.31 R231A decreased apparent affinity for the activator glucose 6-phosphate Zea mays
4.1.1.31 R232Q decreased apparent affinity for the activator glucose 6-phosphate, reduced apparent affinity for the activator glycine Zea mays

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.31 aspartate
-
 Zea mays
4.1.1.31 malate
-
 Zea mays

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.31 Zea mays
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.31
-
 Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.31 phosphoenolpyruvate + HCO3-
-
 Zea mays phosphate + oxaloacetate
-
 ?