Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Platt, A.; Shingler, V.; Taylor, S.C.; Williams, P.A.
    The 4-hydroxy-2-oxovalerate aldolase and acetaldehyde dehydrogenase (acylating) encoded by the nahM and nahO genes of the naphthalene catabolic plasmid pWW60-22 provide further evidence of conservation of meta-cleavage pathway gene sequences (1995), Microbiology, 141, 2223-2233.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.3.39 DNA and amino acid sequence determination and analysis of the gene nahM from the naphthalene catabolic plasmid pWW60-22 of strain NCIMB9816, gene nahM is organized in the nahNLOMK operon of nahOH genes, genetic organization analysis, overview, overexpression in Escherichia coli strain BL21(DE3) using different plasmid vectors, overview Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.39 Mn2+
-
 Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.3.39 4-hydroxy-2-oxovalerate Pseudomonas putida step in a meta pathway pyruvate + acetaldehyde
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.39 Pseudomonas putida P51017 nahKLMO operon; wild-type strain NCIMB9816, gene nahM
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.3.39 0.015 2.3 recombinant enzyme in extracts of different recombinant strains, activity depends on the plasmid vector used for expression and the aldehyde dehydrogenase, EC 1.2.1.10, overview Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.39 4-hydroxy-2-oxovalerate step in a meta pathway Pseudomonas putida pyruvate + acetaldehyde
-
 ?
4.1.3.39 4-hydroxy-2-oxovalerate enzyme acts stereospecifically on the L-enantiomer Pseudomonas putida pyruvate + acetaldehyde
-
 ?
4.1.3.39 additional information the enzyme acts in an enzyme complex with the aldehyde dehydrogenase, EC 1.2.1.10, which acts on acetaldehyde to form acetyl-CoA Pseudomonas putida ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.3.39 HOA
-
 Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.3.39 25
-
 assay at Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.39 8
-
 assay at Pseudomonas putida