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Literature summary extracted from

  • Roy, A.; Guha, N.; Veras, I.D.; Chakraborty, S.; Haldar, D.
    Stimulation of rat liver mitochondrial sn-glycerol-3-phosphate acyltransferase by polymyxin B via enhanced extraction of lysophosphatidic acid (2003), Lipids, 38, 965-972.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.15 Polymyxin B antibiotic, reversible binding, stimulation via enhanced extraction of lysophosphatidic acid, maximal at 60%, the acyl side group does not play a role in stimulation, addition of bovine serum albumin is highly required for stimulation, in absence of bovine serum albumin the antibiotic is slightly inhibitory, addition of Mg2+ enhances the stimulation Rattus norvegicus
2.3.1.15 spermidine activates about 3.5fold in presence of 0.1 mg/ml bovine serum albumin Rattus norvegicus
2.3.1.15 spermine activates about 3fold in presence of 0.1 mg/ml bovine serum albumin Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.15 Polymyxin B in absence of BSA the antibiotic is slightly inhibitory Rattus norvegicus
2.3.1.15 spermidine inhibits slightly in absence of bovine serum albumin Rattus norvegicus
2.3.1.15 spermine inhibits slightly in absence of bovine serum albumin Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.15 endoplasmic reticulum
-
 Rattus norvegicus 5783
-
2.3.1.15 mitochondrial outer membrane
-
 Rattus norvegicus 5741
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.15 Mg2+ activates in presence of polymyxin Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate Rattus norvegicus the enzyme catalyzes the first and committed step in the synthesis of glycerophospholipids and triacylglycerol from sn-glycerol-3-phosphate CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.15 Rattus norvegicus
-
 male Harlan Sprague-Dawley rats
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.15 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.15 additional information
-
-
 Rattus norvegicus
2.3.1.15 0.0027
-
 about, enzyme in presence of 10 mM MgCl2 and 0.1 mg/ml polymyxin Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate the enzyme catalyzes the first and committed step in the synthesis of glycerophospholipids and triacylglycerol from sn-glycerol-3-phosphate Rattus norvegicus CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 palmitoyl-CoA + sn-glycerol 3-phosphate
-
 Rattus norvegicus CoA + 1-palmitoyl-sn-glycerol 3-phosphate i.e. lysophosphatidic acid ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.15 GAT
-
 Rattus norvegicus
2.3.1.15 glycerophosphate acyltransferase
-
 Rattus norvegicus
2.3.1.15 sn-glycerol-3-phosphate acyltransferase
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.15 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.15 7.5
-
 assay at Rattus norvegicus