EC Number | Cloned (Comment) | Organism |
---|---|---|
6.3.1.9 | DNA sequence determination and analysis, expression of wild-type enzyme and mutants in Escherichia coli as His-tagged or Nus-fusion proteins | Crithidia fasciculata |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
6.3.1.9 | R553E | site-directed mutagenesis | Crithidia fasciculata |
6.3.1.9 | R553K | site-directed mutagenesis | Crithidia fasciculata |
6.3.1.9 | R553L | site-directed mutagenesis | Crithidia fasciculata |
6.3.1.9 | R553Q | site-directed mutagenesis | Crithidia fasciculata |
6.3.1.9 | R613E | site-directed mutagenesis | Crithidia fasciculata |
6.3.1.9 | R613K | site-directed mutagenesis | Crithidia fasciculata |
6.3.1.9 | R613L | site-directed mutagenesis | Crithidia fasciculata |
6.3.1.9 | R613Q | site-directed mutagenesis | Crithidia fasciculata |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.3.1.9 | additional information | limited proteolysis with trypsin or factor Xa result in cleavage of wild-type and mutant enzymes at R553 followed by loss of activity, cleavage of wild-type and mutant R553Q can be delayed by presence of substrates | Crithidia fasciculata |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.1.8 | 0.052 | - |
ATP | pH 7.2, 25°C, recombinant trypanothione synthase performing the glutathionylspermidine synthase reaction | Crithidia fasciculata | |
6.3.1.8 | 1.175 | - |
glutathione | pH 7.2, 25°C, recombinant trypanothione synthase performing the glutathionylspermidine synthase reaction | Crithidia fasciculata | |
6.3.1.8 | 7.424 | - |
spermidine | pH 7.2, 25°C, recombinant trypanothione synthase performing the glutathionylspermidine synthase reaction | Crithidia fasciculata | |
6.3.1.9 | additional information | - |
additional information | steady-state kinetics for the multiple enzyme activities, Dalziel equation, overview | Crithidia fasciculata | |
6.3.1.9 | 0.222 | - |
MgATP2- | pH 7.2, 25°C, recombinant trypanothione synthase | Crithidia fasciculata | |
6.3.1.9 | 0.407 | - |
glutathione | pH 7.2, 25°C, recombinant trypanothione synthase | Crithidia fasciculata | |
6.3.1.9 | 0.48 | - |
glutathionylspermidine | pH 7.2, 25°C, recombinant trypanothione synthase | Crithidia fasciculata |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.3.1.8 | Mg2+ | as MgATP2- | Crithidia fasciculata | |
6.3.1.9 | Mg2+ | as MgATP2- | Crithidia fasciculata |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.1.8 | glutathione + spermidine + ATP | Crithidia fasciculata | - |
glutathionylspermidine + ADP + phosphate | - |
? | |
6.3.1.8 | glutathione + spermidine + ATP | Crithidia fasciculata | trypanothione synthase | glutathionylspermidine + ADP + phosphate | - |
? | |
6.3.1.8 | additional information | Crithidia fasciculata | the trypanothione synthase also converts glutathionylspermidine further to trypanothione catalyzing the reaction of EC 6.3.1.9 | ? | - |
? | |
6.3.1.9 | glutathione + glutathionylspermidine + ATP | Crithidia fasciculata | - |
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate | - |
? | |
6.3.1.9 | additional information | Crithidia fasciculata | the trypanothione synthase also catalyzes the reaction of glutathionylspermidine synthase, EC 6.3.1.8 | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.3.1.8 | Crithidia fasciculata | P90518 | - |
- |
6.3.1.8 | Crithidia fasciculata | Q5DM89 | trypanothione synthase; a dual specificity enzyme | - |
6.3.1.9 | Crithidia fasciculata | Q5DM89 | trypanothione synthase; a dual specificity enzyme | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.3.1.9 | recombinant His-tagged wild-type enzyme and mutants from Escherichia coli by nickel affinity chromatography | Crithidia fasciculata |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
6.3.1.8 | glutathione + spermidine + ATP = glutathionylspermidine + ADP + phosphate | the reaction is catalyzed by trypanothione synthase and glutathionylspermidine synthase in Crithidia fasciculata | Crithidia fasciculata | |
6.3.1.8 | glutathione + spermidine + ATP = glutathionylspermidine + ADP + phosphate | the reaction is catalyzed by trypanothione synthase, EC 6.3.1.9, and glutathionylspermidine synthase, EC 6.3.1.8, in Crithidia fasciculata | Crithidia fasciculata | |
6.3.1.9 | glutathione + glutathionylspermidine + ATP = N1,N8-bis(glutathionyl)spermidine + ADP + phosphate | the reaction is catalyzed by trypanothione synthase, which also catalyzes the reaction of glutathionyspermidine synthase, EC 6.3.1.8, in Crithidia fasciculata, trypanothione synthase further exhibits amidase and marginal ATPase activities | Crithidia fasciculata |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
6.3.1.8 | 2 | - |
about, purified recombinant trypanothione synthase performing the glutathionylspermidine synthase reaction | Crithidia fasciculata |
6.3.1.9 | additional information | - |
- |
Crithidia fasciculata |
6.3.1.9 | 2.1 | 2.2 | purified recombinant trypanothione synthase performing the trypanothione synthase reaction, average of the activity of recombinant enzymes expressed from different constructs, overview | Crithidia fasciculata |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.1.8 | 2 glutathione + spermidine + 2 ATP | trypanothione synthase overall reaction | Crithidia fasciculata | N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate | - |
? | |
6.3.1.8 | glutathione + spermidine + ATP | - |
Crithidia fasciculata | glutathionylspermidine + ADP + phosphate | - |
? | |
6.3.1.8 | glutathione + spermidine + ATP | trypanothione synthase | Crithidia fasciculata | glutathionylspermidine + ADP + phosphate | - |
? | |
6.3.1.8 | glutathione + spermidine + ATP | specific for, the enzyme is distinct from the trypanothione synthase, which also catalyzes the reaction but converts glutathionylsperimidine further to trypanothione | Crithidia fasciculata | glutathionylspermidine + ADP + phosphate | - |
? | |
6.3.1.8 | additional information | the trypanothione synthase also converts glutathionylspermidine further to trypanothione catalyzing the reaction of EC 6.3.1.9 | Crithidia fasciculata | ? | - |
? | |
6.3.1.9 | 2 glutathione + spermidine + 2 ATP | trypanothione synthase overall reaction | Crithidia fasciculata | N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate | - |
? | |
6.3.1.9 | glutathione + glutathionylspermidine + ATP | - |
Crithidia fasciculata | N1,N8-bis(glutathionyl)spermidine + ADP + phosphate | - |
? | |
6.3.1.9 | glutathione + glutathionylspermidine + ATP | dynamic interactions of remote domains in substrate binding and catalysis | Crithidia fasciculata | N1,N8-bis(glutathionyl)spermidine + ADP + phosphate | - |
? | |
6.3.1.9 | glutathione + N8-acetylspermidine + ATP | - |
Crithidia fasciculata | N1-glutathionyl-N8-acetylspermidine + ADP + phosphate | - |
? | |
6.3.1.9 | glutathione + spermidine + ATP | ATP in form of MgATP2- | Crithidia fasciculata | glutathionylspermidine + ADP + phosphate | - |
? | |
6.3.1.9 | additional information | the trypanothione synthase also catalyzes the reaction of glutathionylspermidine synthase, EC 6.3.1.8 | Crithidia fasciculata | ? | - |
? | |
6.3.1.9 | additional information | the trypanothione synthase also catalyzes the reaction of glutathionylspermidine synthase, EC 6.3.1.8, and exhibits amidase and marginal ATPase activities | Crithidia fasciculata | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.3.1.8 | GspS | - |
Crithidia fasciculata |
6.3.1.8 | TryS | - |
Crithidia fasciculata |
6.3.1.9 | TryS | - |
Crithidia fasciculata |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.3.1.8 | 25 | - |
assay at | Crithidia fasciculata |
6.3.1.9 | 25 | - |
assay at | Crithidia fasciculata |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.1.8 | 4.2 | - |
spermidine | pH 7.2, 25°C, recombinant trypanothione synthase performing the glutathionylspermidine synthase reaction | Crithidia fasciculata | |
6.3.1.9 | additional information | - |
additional information | - |
Crithidia fasciculata | |
6.3.1.9 | 8.7 | - |
glutathionylspermidine | pH 7.2, 25°C, recombinant trypanothione synthase | Crithidia fasciculata |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.3.1.8 | 7.2 | - |
assay at | Crithidia fasciculata |
6.3.1.9 | 7.2 | 7.5 | assay at | Crithidia fasciculata |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
6.3.1.8 | ATP | as MgATP2- | Crithidia fasciculata | |
6.3.1.9 | ATP | as MgATP2- | Crithidia fasciculata |