EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.1.32 | native and mutant enzymes expressed as His-tag fusion protein in Sf9 cells | Caenorhabditis elegans |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.1.32 | C252S | slightly increased turnover | Caenorhabditis elegans |
2.7.1.32 | D255A | no activity detectable | Caenorhabditis elegans |
2.7.1.32 | D255E | activity almost completely abolished | Caenorhabditis elegans |
2.7.1.32 | D255N | no activity detectable | Caenorhabditis elegans |
2.7.1.32 | D301A | no activity detectable | Caenorhabditis elegans |
2.7.1.32 | D301E | no activity detectable | Caenorhabditis elegans |
2.7.1.32 | D301N | no activity detectable | Caenorhabditis elegans |
2.7.1.32 | D313A | low expression level | Caenorhabditis elegans |
2.7.1.32 | E125A | reduced activity | Caenorhabditis elegans |
2.7.1.32 | E125D | increased turnover | Caenorhabditis elegans |
2.7.1.32 | E125Q | reduced activity | Caenorhabditis elegans |
2.7.1.32 | E134A | slightly increased turnover | Caenorhabditis elegans |
2.7.1.32 | E151A | slightly increased turnover | Caenorhabditis elegans |
2.7.1.32 | E303A | strongly reduced activity, increased Km for both substrates, strongly increased Km for Mg2+ | Caenorhabditis elegans |
2.7.1.32 | E303D | reduced activity | Caenorhabditis elegans |
2.7.1.32 | E303Q | reduced activity | Caenorhabditis elegans |
2.7.1.32 | E320A | slightly increased turnover | Caenorhabditis elegans |
2.7.1.32 | H181A | low expression level | Caenorhabditis elegans |
2.7.1.32 | H253A | reduced activity | Caenorhabditis elegans |
2.7.1.32 | N260A | activity almost completely abolished, strongly increased Km for Mg2+ | Caenorhabditis elegans |
2.7.1.32 | N308A | reduced activity | Caenorhabditis elegans |
2.7.1.32 | N308D | slightly increased turnover | Caenorhabditis elegans |
2.7.1.32 | N308Q | reduced activity | Caenorhabditis elegans |
2.7.1.32 | N354A | strongly reduced activity, only minor effect on Km for both substrates | Caenorhabditis elegans |
2.7.1.32 | N87A | reduced activity | Caenorhabditis elegans |
2.7.1.32 | R111A | reduced activity | Caenorhabditis elegans |
2.7.1.32 | R149A | reduced activity | Caenorhabditis elegans |
2.7.1.32 | R149K | slightly increased turnover | Caenorhabditis elegans |
2.7.1.32 | S85T | reduced activity | Caenorhabditis elegans |
2.7.1.32 | S86A | strongly reduced activity, increased Km for ATP | Caenorhabditis elegans |
2.7.1.32 | W387A | strongly reduced activity, only minor effect on Km for both substrates | Caenorhabditis elegans |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.32 | Ca2+ | - |
Caenorhabditis elegans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.32 | 0.4 | - |
choline | N260A mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 0.7 | - |
choline | R149A mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 0.7 | - |
choline | S85T mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 0.8 | - |
choline | E125D mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 0.8 | - |
choline | N308Q mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 1 | - |
ATP | C252S mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 1 | - |
choline | C252S mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 1 | - |
choline | N308D mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 1 | - |
ATP | N308Q mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 1 | - |
choline | N354A mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 1 | - |
choline | wild type enzyme including His-tag, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 1.1 | - |
choline | E125A mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 1.2 | - |
ATP | N354A mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 1.2 | - |
choline | R149K mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 1.2 | - |
choline | W387A mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 1.3 | - |
ATP | D255D mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 1.5 | - |
choline | N308A mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 1.5 | - |
ATP | N308D mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 1.6 | - |
choline | E125Q mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 1.6 | - |
choline | H253A mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 1.6 | - |
choline | wild type enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 1.7 | - |
ATP | E134A mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 1.7 | - |
ATP | N87A mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 1.7 | - |
choline | R111A mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 1.7 | - |
ATP | wild type enzyme including His-tag, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 1.8 | - |
choline | E134A mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 1.8 | - |
choline | N87A mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 1.9 | - |
ATP | H253A mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 1.9 | - |
ATP | S85T mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 2 | - |
ATP | N308A mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 2.2 | - |
ATP | E125D mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 2.2 | - |
choline | E151A mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 2.2 | - |
ATP | R149A mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 2.2 | - |
ATP | W387A mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 2.3 | - |
ATP | R149K mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 2.4 | - |
ATP | wild type enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 2.5 | - |
ATP | S86A mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 3 | - |
choline | E320A mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 3.1 | - |
ATP | N260A mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 3.2 | - |
ATP | E125A mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 3.4 | - |
choline | E303Q mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 3.6 | - |
choline | E303D mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 4.2 | - |
ATP | E125Q mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 4.5 | - |
ATP | E151A mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 5 | - |
choline | S86A mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 5.4 | - |
choline | D255D mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 5.4 | - |
ATP | E303A mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 6.9 | - |
ATP | E303D mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 7 | - |
ATP | R111A mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 8.8 | - |
ATP | E303Q mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 10 | - |
choline | E303A mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 45 | - |
ATP | E320A mutant enzyme, pH 10, 37°C | Caenorhabditis elegans |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.32 | Mg2+ | - |
Caenorhabditis elegans |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.1.32 | 51000 | - |
2 * 51000, recombinant enzyme including His-tag, SDS-PAGE, native mass by gel filtration | Caenorhabditis elegans |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.32 | ATP + choline | Caenorhabditis elegans | initial step of the CDP-choline pathway, that yields phosphatidylcholine as final product, involved in mitogenic signaling transduction | ADP + O-phosphocholine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.32 | Caenorhabditis elegans | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.32 | recombinant enzymes using His-tag | Caenorhabditis elegans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.32 | ATP + choline | - |
Caenorhabditis elegans | ADP + O-phosphocholine | - |
? | |
2.7.1.32 | ATP + choline | initial step of the CDP-choline pathway, that yields phosphatidylcholine as final product, involved in mitogenic signaling transduction | Caenorhabditis elegans | ADP + O-phosphocholine | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.1.32 | dimer | 2 * 51000, recombinant enzyme including His-tag, SDS-PAGE, native mass by gel filtration | Caenorhabditis elegans |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.1.32 | More | at least four genes encoding active choline kinase | Caenorhabditis elegans |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.32 | 0.24 | - |
choline | N260A mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 0.25 | - |
choline | D255D mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 2.4 | - |
choline | W387A mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 2.6 | - |
choline | N354A mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 7 | - |
choline | E303A mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 9.8 | - |
choline | S86A mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 20.8 | - |
choline | N308A mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 21 | - |
choline | E125A mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 27.1 | - |
choline | R111A mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 29.7 | - |
choline | N87A mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 31.4 | - |
choline | E125Q mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 35.7 | - |
choline | S85T mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 37.5 | - |
choline | N308Q mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 38.7 | - |
choline | E303D mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 38.9 | - |
choline | R149A mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 42.9 | - |
choline | H253A mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 58.7 | - |
choline | E303Q mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 72.3 | - |
choline | wild type enzyme including His-tag, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 74 | - |
choline | wild type enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 79.9 | - |
choline | E320A mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 84.3 | - |
choline | C252S mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 84.6 | - |
choline | N308D mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 87.9 | - |
choline | R149K mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 89.2 | - |
choline | E151A mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 97.3 | - |
choline | E134A mutant enzyme, pH 10, 37°C | Caenorhabditis elegans | |
2.7.1.32 | 130 | - |
choline | E125D mutant enzyme, pH 10, 37°C | Caenorhabditis elegans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.32 | 10 | - |
- |
Caenorhabditis elegans |