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Literature summary extracted from

  • Ariyanayagam, M.R.; Oza, S.L.; Guther, M.L.S.; Fairlamb, A.H.
    Phenotypic analysis of trypanothione synthetase knockdown in the African trypanosome (2005), Biochem. J., 391, 425-432.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.1.8 DNA sequence determination, cloning and RNAi transfection, expression in Escherichia coli Trypanosoma brucei
6.3.1.9 DNA sequence determination, cloning and RNAi transfection, expression in Escherichia coli Trypanosoma brucei

Protein Variants

EC Number Protein Variants Comment Organism
6.3.1.8 additional information enzyme downregulation leads to decreased growth rate of the parasite, increased H2O2 and tert-butylhydroperoxide sensitivity, enzyme knockdown leads to depletion of both trypanothione and glutathionylspermidine, and glutathione and polyamine accumulation, RNA interference method, overview Trypanosoma brucei
6.3.1.9 additional information enzyme downregulation leads to decreased growth rate of the parasite, increased H2O2 and tert-butylhydroperoxide sensitivity, enzyme knockdown leads to depletion of both trypanothione and glutathionylspermidine, and glutathione and polyamine accumulation, RNA interference method, overview Trypanosoma brucei

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.3.1.8 kinetoplast
-
 Trypanosoma brucei 20023
-
6.3.1.9 kinetoplast
-
 Trypanosoma brucei 20023
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.1.8 glutathione + spermidine + ATP Trypanosoma brucei trypanothione synthase catalyzes both the reaction of glutathionylspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9, in Trypanosoma brucei glutathionylspermidine + ADP + phosphate
-
 ?
6.3.1.8 additional information Trypanosoma brucei important reaction in defense against chemical and oxidant stress, thiol redox homeostasis, ribonucleotide metabolism, and drug resistance in parasitic kinetoplastids ?
-
 ?
6.3.1.9 2 glutathione + spermidine + 2 ATP Trypanosoma brucei important reaction in defense against chemical and oxidant stress, thiol redox homeostasis, ribonucleotide metabolism, and drug resistance in parasitic kinetoplastids N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate
-
 ?
6.3.1.9 glutathione + glutathionylspermidine + ATP Trypanosoma brucei trypanothione synthase catalyzes both the reaction of glutathionyspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9, in Trypanosoma brucei N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
 ?
6.3.1.9 glutathione + spermidine + ATP Trypanosoma brucei trypanothione synthase catalyzes both the reaction of glutathionyspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9, in Trypanosoma brucei glutathionylspermidine + ADP + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.8 Trypanosoma brucei
-
 bifunctional enzyme with glutathionylspermidine synthase and trypanothione synthase activity
-
6.3.1.9 Trypanosoma brucei
-
 bifunctional enzyme with glutathionylspermidine synthase and trypanothione synthase activity
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.8 glutathione + spermidine + ATP trypanothione synthase catalyzes both the reaction of glutathionylspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9, in Trypanosoma brucei Trypanosoma brucei glutathionylspermidine + ADP + phosphate
-
 ?
6.3.1.8 additional information important reaction in defense against chemical and oxidant stress, thiol redox homeostasis, ribonucleotide metabolism, and drug resistance in parasitic kinetoplastids Trypanosoma brucei ?
-
 ?
6.3.1.9 2 glutathione + spermidine + 2 ATP important reaction in defense against chemical and oxidant stress, thiol redox homeostasis, ribonucleotide metabolism, and drug resistance in parasitic kinetoplastids Trypanosoma brucei N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate
-
 ?
6.3.1.9 2 glutathione + spermidine + 2 ATP trypanothione synthase overall reaction Trypanosoma brucei N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate
-
 ?
6.3.1.9 glutathione + glutathionylspermidine + ATP trypanothione synthase catalyzes both the reaction of glutathionyspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9, in Trypanosoma brucei Trypanosoma brucei N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
 ?
6.3.1.9 glutathione + spermidine + ATP trypanothione synthase catalyzes both the reaction of glutathionyspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9, in Trypanosoma brucei Trypanosoma brucei glutathionylspermidine + ADP + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.1.9 TryS
-
 Trypanosoma brucei

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.1.8 ATP
-
 Trypanosoma brucei
6.3.1.9 ATP
-
 Trypanosoma brucei