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Literature summary extracted from

  • Takeuchi, R.; Oshige, M.; Uchida, M.; Ishikawa, G.; Takata, K.; Shimanouchi, K.; Kanai, Y.; Ruike, T.; Morioka, H.; Sakaguchi, K.
    Purification of Drosophila DNA polymerase zeta by REV1 protein-affinity chromatography (2004), Biochem. J., 382, 535-543.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.7 aphidicolin IC50: 0.01 mM Drosophila melanogaster
2.7.7.7 KCl 150-200 mM Drosophila melanogaster
2.7.7.7 additional information enzyme is resistant to NEM and 2',3'-dideoxythymidine 5-triphosphate Drosophila melanogaster

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.7 Mg2+ divalent cation required, optimal concentration is 5 mm Drosophila melanogaster

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.7 240000
-
 gel filtration Drosophila melanogaster

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.7 Drosophila melanogaster
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.7
-
 Drosophila melanogaster

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.7 embryo
-
 Drosophila melanogaster
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.7 additional information
-
-
 Drosophila melanogaster

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.7 deoxynucleoside triphosphate + DNAn preference for poly(dA)/oligo(dT)10:1 as a template primer and has high processivity for DNA synthesis Drosophila melanogaster diphosphate + DNAn+1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.7 Dmpol zeta
-
 Drosophila melanogaster
2.7.7.7 DNA polymerase zeta
-
 Drosophila melanogaster

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.7 7.5
-
-
 Drosophila melanogaster

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.7 6.5 8.5 about 50% of maximal activity at pH 6.5 and 8.5 Drosophila melanogaster

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.7.7 0.01
-
 IC50: 0.01 mM Drosophila melanogaster aphidicolin