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Literature summary extracted from

  • Duran, R.; Villarino, A.; Bellinzoni, M.; Wehenkel, A.; Fernandez, P.; Boitel, B.; Cole, S.T.; Alzari, P.M.; Cervenansky, C.
    Conserved autophosphorylation pattern in activation loops and juxtamembrane regions of Mycobacterium tuberculosis Ser/Thr protein kinases (2005), Biochem. Biophys. Res. Commun., 333, 858-867.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.1 additional information autophosphorylation patterns for activation of PknB, PknD, PknE, and PknF, e.g. PknB phosphorylation at Thr171 and Thr173 of the activation loop and at Thr294 of the juxtamembrane domain are necessary for activity Mycobacterium tuberculosis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.1 expression of His-tagged PknB, PknD, PknE, and PknF in Escherichia coli strain BL21(DE3) Mycobacterium tuberculosis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.1 membrane
-
 Mycobacterium tuberculosis 16020
-
2.7.11.1 additional information PknB, PknD, PknE, and PknF possess an N-terminal kinase domain, a juxtamembrane domain of varying length, and an activation loop Mycobacterium tuberculosis
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.1 Mn2+
-
 Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.1 Mycobacterium tuberculosis
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.11.1 phosphoprotein PknB, PknD, PknE, and PknF are autophosphorylated at multiple sites, phosphorylation patterns, overview, the enzymes are dephosphorylated by the cognate mycobacterial phosphatase PstP Mycobacterium tuberculosis

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.1 recombinant His-tagged PknB, PknD, PknE, and PknF rom Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.1 ATP + myelin basic protein PknB, PknD, PknE, and PknF Mycobacterium tuberculosis ADP + phosphorylated myelin basic protein
-
 ?
2.7.11.1 additional information PknB, PknD, PknE, and PknF perform autophosphorylation Mycobacterium tuberculosis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.1 More PknB, PknD, PknE, and PknF possess an N-terminal kinase domain, a juxtamembrane domain of varying length, and an activation loop Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.7.11.1 PknB
-
 Mycobacterium tuberculosis
2.7.11.1 PknD
-
 Mycobacterium tuberculosis
2.7.11.1 PknE
-
 Mycobacterium tuberculosis
2.7.11.1 PknF
-
 Mycobacterium tuberculosis
2.7.11.1 Ser/Thr protein kinase
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.1 30
-
 assay at Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.1 7
-
 assay at Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.1 ATP
-
 Mycobacterium tuberculosis