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Literature summary extracted from

  • Fukamachi, H.; Nakano, Y.; Okano, S.; Shibata, Y.; Abiko, Y.; Yamashita, Y.
    High production of methyl mercaptan by L-methionine-alpha-deamino-gamma-mercaptomethane lyase from Treponema denticola (2005), Biochem. Biophys. Res. Commun., 331, 127-131.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.4.1.11 drug development methyl mercaptan may not only cause oral malodour but also play an important role in the pathogenicity of Treptonema denticola. The enzyme may be an exploitable target for novel chemotherapeutic drugs. An inhibitor of METase may represent a new class of compounds with the potential for preventing and treating oral malodor Treponema denticola

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.11 expression in Escherichia coli Treponema denticola

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.11 0.55
-
 L-methionine
-
 Treponema denticola

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.11 43500
-
 SDS-PAGE Treponema denticola

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.11 L-methionine + H2O Porphyromonas gingivalis
-
 methanethiol + NH3 + 2-oxobutanoate
-
 ?
4.4.1.11 L-methionine + H2O Treponema denticola
-
 methanethiol + NH3 + 2-oxobutanoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.11 Porphyromonas gingivalis
-
-
-
4.4.1.11 Treponema denticola
-
 ATCC 35405
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.11
-
 Treponema denticola

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.11 36.6
-
-
 Treponema denticola

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.11 L-homocysteine + H2O
-
 Treponema denticola hydrogen sulfide + NH3 + 2-oxobutanoate
-
 ?
4.4.1.11 L-methionine + H2O
-
 Porphyromonas gingivalis methanethiol + NH3 + 2-oxobutanoate
-
 ?
4.4.1.11 L-methionine + H2O
-
 Treponema denticola methanethiol + NH3 + 2-oxobutanoate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.4.1.11 L-methionine-alpha-deamino-gamma-mercaptomethane lyase
-
 Porphyromonas gingivalis
4.4.1.11 L-methionine-alpha-deamino-gamma-mercaptomethane lyase
-
 Treponema denticola
4.4.1.11 METase
-
 Porphyromonas gingivalis
4.4.1.11 METase
-
 Treponema denticola