Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Taguchi, G.; Ubukata, T.; Hayashida, N.; Yamamoto, H.; Okazaki, M.
    Cloning and characterization of a glucosyltransferase that reacts on 7-hydroxyl group of flavonol and 3-hydroxylgroup of coumarin from tobacco cells (2003), Arch. Biochem. Biophys., 420, 95-102.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.237 additional information enzyme expression is inducible by plant hormones, such as salicylic acid, auxin, 2,4-dichorophenoxyacetic acid, and jasmonate Nicotiana tabacum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.237 DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Nicotiana tabacum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.237 0.0065
-
 kaempferol pH 8.0, 30°C, recombinant enzyme Nicotiana tabacum
2.4.1.237 0.0236
-
 3-hydroxycoumarin pH 8.0, 30°C, recombinant enzyme Nicotiana tabacum
2.4.1.237 0.0432
-
 UDP-glucose pH 8.0, 30°C, recombinant enzyme Nicotiana tabacum

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.237 Nicotiana tabacum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.237 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Nicotiana tabacum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.237 additional information
-
-
 Nicotiana tabacum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.237 additional information broad substrate specificity, no activity with salicylic acid, cyanidin, and cyanidin 3-O-glucoside, poor activity with 4-hydroxycoumarin and 5-hydroxyflavone Nicotiana tabacum ?
-
 ?
2.4.1.237 UDP-alpha-D-glucose + kaempferol preferred substrate Nicotiana tabacum UDP + kaempferol 7-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-alpha-D-glucose + quercetin 19% of the activity with kaempferol Nicotiana tabacum UDP + quercetin 7-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + 1-naphthol 44% of the activity with kaempferol Nicotiana tabacum UDP + naphthol 1-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + 2-coumaric acid 41% of the activity with kaempferol Nicotiana tabacum UDP + ?
-
 ?
2.4.1.237 UDP-glucose + 2-naphthol 14% of the activity with kaempferol Nicotiana tabacum UDP + naphthol 2-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + 3-hydroxycoumarin 310% of the activity with kaempferol Nicotiana tabacum UDP + coumarin 3-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + 3-hydroxyflavone 12% of the activity with kaempferol Nicotiana tabacum UDP + 3-hydroxyflavone 7-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + 4-coumaric acid 43% of the activity with kaempferol Nicotiana tabacum UDP + ?
-
 ?
2.4.1.237 UDP-glucose + 6-hydroxyflavone 24% of the activity with kaempferol Nicotiana tabacum UDP + 6-hydroxyflavone 7-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + 7-hydroxyflavone 53% of the activity with kaempferol Nicotiana tabacum UDP + 7-hydroxyflavone 7-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + esculetin 24% of the activity with kaempferol Nicotiana tabacum UDP + esculetin 7-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + kaempferol 3-O-glucoside 45% of the activity with kaempferol Nicotiana tabacum UDP + kaempferol 3,7beta-O-diglucoside
-
 ?
2.4.1.237 UDP-glucose + scopoletin 11% of the activity with kaempferol Nicotiana tabacum UDP + scopoletin 7-O-beta-D-glucoside
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.237 GT2
-
 Nicotiana tabacum
2.4.1.237 NTGT2
-
 Nicotiana tabacum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.237 30
-
 assay at Nicotiana tabacum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.237 8
-
 with kaempferol as substrate Nicotiana tabacum