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Literature summary extracted from

  • Ferry, G.; Ubeaud, C.; Dauly, C.; Mozo, J.; Guillard, S.; Berger, S.; Jimenez, S.; Scoul, C.; Leclerc, G.; Yous, S.; Delagrange, P.; Boutin, J.A.
    Purification of the recombinant human serotonin N-acetyltransferase (EC 2.3.1.87): further characterization of and comparison with AANAT from other species (2004), Protein Expr. Purif., 38, 84-98.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.87 baculovirus infection of Tn5 insect cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.87 acetyl-CoA-tryptamine IC50: 0.00041 mM, GST-AANAT fusion protein Homo sapiens
2.3.1.87 acetyl-CoA-tryptamine IC50: 0.00062 mM, GST-AANAT fusion protein Ovis aries
2.3.1.87 acetyl-CoA-tryptamine IC50: 0.0008 mM, GST-AANAT fusion protein Rattus norvegicus
2.3.1.87 bromoacetyltryptamine IC50: 0.00143 mM, GST-AANAT fusion protein Homo sapiens
2.3.1.87 bromoacetyltryptamine IC50: 0.00128 mM, GST-AANAT fusion protein Ovis aries
2.3.1.87 bromoacetyltryptamine IC50: 0.0051 mM, GST-AANAT fusion protein Rattus norvegicus
2.3.1.87 S 20251 IC50: 0.002 mM, GST-AANAT fusion protein Homo sapiens
2.3.1.87 S 20251 IC50: 0.0013 mM, GST-AANAT fusion protein Ovis aries
2.3.1.87 S 20251 IC50: 0.0024 mM, GST-AANAT fusion protein Rattus norvegicus
2.3.1.87 S 23823 IC50: 0.005 mM, GST-AANAT fusion protein Homo sapiens
2.3.1.87 S 23823 IC50: 0.031 mM, GST-AANAT fusion protein Rattus norvegicus
2.3.1.87 S 27244 IC50: 0.00072 mM, GST-AANAT fusion protein Homo sapiens
2.3.1.87 S 27244 IC50: 0.00026 mM, GST-AANAT fusion protein Ovis aries
2.3.1.87 S 27244 IC50: 0.00036 mM, GST-AANAT fusion protein Rattus norvegicus
2.3.1.87 S 27481 IC50: 0.00018 mM, GST-AANAT fusion protein Homo sapiens
2.3.1.87 S 27481 IC50: 0.0004 mM, GST-AANAT fusion protein Ovis aries
2.3.1.87 S 27481 IC50: 0.0004 mM, GST-AANAT fusion protein Rattus norvegicus
2.3.1.87 S 28036 IC50: 0.0056 mM,GST-AANAT fusion protein Homo sapiens
2.3.1.87 S 28036 IC50: 0.00073 mM, GST-AANAT fusion protein Ovis aries
2.3.1.87 S 28036 IC50: 0.0017 mM, GST-AANAT fusion protein Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.87 0.17
-
 2-Phenylethylamine pH 6.8, 37°C Homo sapiens
2.3.1.87 0.265
-
 acetyl-CoA pH 6.8, 37°C Ovis aries
2.3.1.87 0.34
-
 2-(2,3-dichlorophenyl)-ethylamine pH 6.8, 37°C Homo sapiens
2.3.1.87 0.47
-
 2,5-dimethoxyphenylethylamine pH 6.8, 37°C Ovis aries
2.3.1.87 0.49
-
 2-methoxyphenylethylamine pH 6.8, 37°C Ovis aries
2.3.1.87 0.53
-
 acetyl-CoA pH 6.8, 37°C Homo sapiens
2.3.1.87 0.62
-
 2-(3-chlorophenyl)-ethylamine pH 6.8, 37°C Homo sapiens
2.3.1.87 0.64
-
 serotonin pH 6.8, 37°C Ovis aries
2.3.1.87 0.65
-
 acetyl-CoA pH 6.8, 37°C Rattus norvegicus
2.3.1.87 0.75
-
 2-(4-bromophenyl)-ethylamine pH 6.8, 37°C Homo sapiens
2.3.1.87 0.9
-
 2-(2-chlorophenyl)-ethylamine pH 6.8, 37°C Ovis aries
2.3.1.87 0.98
-
 2-(p-fluorophenyl)-ethylamine pH 6.8, 37°C Homo sapiens
2.3.1.87 1.1
-
 2-(3-fluorophenyl)-ethylamine pH 6.8, 37°C Homo sapiens
2.3.1.87 1.17
-
 2-(p-tolyl)-ethylamine pH 6.8, 37°C Homo sapiens
2.3.1.87 1.28
-
 2-(4-fluorophenyl)-ethylamine pH 6.8, 37°C Ovis aries
2.3.1.87 1.3
-
 2-methoxyphenylethylamine pH 6.8, 37°C Homo sapiens
2.3.1.87 1.31
-
 2-(3-chlorophenyl)-ethylamine pH 6.8, 37°C Ovis aries
2.3.1.87 1.35
-
 serotonin pH 6.8, 37°C Homo sapiens
2.3.1.87 1.37
-
 2-fluorophenylethylamine pH 6.8, 37°C Ovis aries
2.3.1.87 1.37
-
 2-fluorophenylethylamine pH 6.8, 37°C Homo sapiens
2.3.1.87 1.405
-
 2-(p-nitrophenyl)-ethylamine pH 6.8, 37°C Ovis aries
2.3.1.87 1.55
-
 2-(4-bromophenyl)-ethylamine pH 6.8, 37°C Ovis aries
2.3.1.87 1.58
-
 2-(p-tolyl)-ethylamine pH 6.8, 37°C Ovis aries
2.3.1.87 1.65
-
 2-(3-fluorophenyl)-ethylamine pH 6.8, 37°C Ovis aries
2.3.1.87 1.7
-
 serotonin pH 6.8, 37°C Rattus norvegicus
2.3.1.87 1.76
-
 2-(3,4-dihydroxyphenyl)-ethylamine pH 6.8, 37°C Ovis aries
2.3.1.87 1.8
-
 2-Phenylethylamine pH 6.8, 37°C Ovis aries
2.3.1.87 1.815
-
 3-methoxytyramine pH 6.8, 37°C Ovis aries
2.3.1.87 1.9
-
 6-Hydroxydopamine pH 6.8, 37°C Homo sapiens
2.3.1.87 1.91
-
 2-(p-fluorophenyl)-ethylamine pH 6.8, 37°C Ovis aries
2.3.1.87 2.07
-
 3-methoxy-2-phenylethylamine pH 6.8, 37°C Ovis aries
2.3.1.87 2.2
-
 2-(4-fluorophenyl)-ethylamine pH 6.8, 37°C Homo sapiens
2.3.1.87 2.33
-
 2-(4-chlorophenyl)ethylamine pH 6.8, 37°C Ovis aries
2.3.1.87 2.74
-
 2-(2-chlorophenyl)-ethylamine pH 6.8, 37°C Homo sapiens
2.3.1.87 3.46
-
 2-(2,3-dichlorophenyl)-ethylamine pH 6.8, 37°C Ovis aries
2.3.1.87 9.5
-
 2-Phenylethylamine pH 6.8, 37°C Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.87 Homo sapiens Q16613
-
-
2.3.1.87 Ovis aries
-
-
-
2.3.1.87 Rattus norvegicus Q64666
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.87 denaturation/renaturation process in which the enzyme is immobilized on an affinity chromatography column Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.87 7.17
-
-
 Ovis aries
2.3.1.87 10.57
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.87 acetyl-CoA + 2,5-dimethoxyphenylethylamine 24% of the activity with 2-phenylethylamine Homo sapiens CoA + ?
-
 ?
2.3.1.87 acetyl-CoA + 2,5-dimethoxyphenylethylamine 68% of the activity with 2-phenylethylamine Ovis aries CoA + N-acetyl-2,5-dimethoxyphenylethylamine
-
 ?
2.3.1.87 acetyl-CoA + 2-(2,3-dichlorophenyl)-ethylamine 78% of the activity with 2-phenylethylamine Homo sapiens CoA + N-acetyl-2-(2,3-dichlorophenyl)-ethylamine
-
 ?
2.3.1.87 acetyl-CoA + 2-(2,3-dichlorophenyl)-ethylamine 84% of the activity with 2-phenylethylamine Ovis aries CoA + N-acetyl-2-(2,3-dichlorophenyl)-ethylamine
-
 ?
2.3.1.87 acetyl-CoA + 2-(2-chlorophenyl)-ethylamine 102% of the activity with 2-phenylethylamine Ovis aries CoA + N-acetyl-2-(2-chlorophenyl)-ethylamine
-
 ?
2.3.1.87 acetyl-CoA + 2-(2-chlorophenyl)-ethylamine 130% of the activity with 2-phenylethylamine Homo sapiens CoA + N-acetyl-2-(2-chlorophenyl)-ethylamine
-
 ?
2.3.1.87 acetyl-CoA + 2-(3,4-dihydroxyphenyl)-ethylamine 64% of the activity with 2-phenylethylamine Ovis aries CoA + N-acetyl-2-(3,4-dihydroxyphenyl)-ethylamine
-
 ?
2.3.1.87 acetyl-CoA + 2-(3,4-dihydroxyphenyl)-ethylamine 7% of the activity with 2-phenylethylamine Homo sapiens CoA + N-acetyl-2-(3,4-dihydroxyphenyl)-ethylamine
-
 ?
2.3.1.87 acetyl-CoA + 2-(3,4-dimethoxyphenyl)-ethylamine 15% of the activity with 2-phenylethylamine Ovis aries CoA + N-acetyl-2-(3,4-dimethoxyphenyl)-ethylamine
-
 ?
2.3.1.87 acetyl-CoA + 2-(3,4-dimethoxyphenyl)-ethylamine 4.4% of the activity with 2-phenylethylamine Homo sapiens CoA + N-acetyl-2-(3,4-dimethoxyphenyl)-ethylamine
-
 ?
2.3.1.87 acetyl-CoA + 2-(3-chlorophenyl)-ethylamine 110% of the activity with 2-phenylethylamine Ovis aries CoA + N-acetyl-2-(3-chlorophenyl)-ethylamine
-
 ?
2.3.1.87 acetyl-CoA + 2-(3-chlorophenyl)-ethylamine 67% of the activity with 2-phenylethylamine Homo sapiens CoA + N-acetyl-2-(3-chlorophenyl)-ethylamine
-
 ?
2.3.1.87 acetyl-CoA + 2-(3-fluorophenyl)-ethylamine 65% of the activity with 2-phenylethylamine Homo sapiens CoA + N-acetyl-2-(3-fluorophenyl)-ethylamine
-
 ?
2.3.1.87 acetyl-CoA + 2-(3-fluorophenyl)-ethylamine 93% of the activity with 2-phenylethylamine Ovis aries CoA + N-acetyl-2-(3-fluorophenyl)-ethylamine
-
 ?
2.3.1.87 acetyl-CoA + 2-(4-bromophenyl)-ethylamine 48% of the activity with 2-phenylethylamine Homo sapiens CoA + N-acetyl-2-(4-bromophenyl)-ethylamine
-
 ?
2.3.1.87 acetyl-CoA + 2-(4-bromophenyl)-ethylamine 87% of the activity with 2-phenylethylamine Ovis aries CoA + N-acetyl-2-(4-bromophenyl)-ethylamine
-
 ?
2.3.1.87 acetyl-CoA + 2-(4-chlorophenyl)ethylamine 68% of the activity with 2-phenylethylamine Homo sapiens CoA + N-acetyl-2-(4-chlorophenyl)ethylamine
-
 ?
2.3.1.87 acetyl-CoA + 2-(4-chlorophenyl)ethylamine 91% of the activity with 2-phenylethylamine Ovis aries CoA + N-acetyl-2-(4-chlorophenyl)ethylamine
-
 ?
2.3.1.87 acetyl-CoA + 2-(4-fluorophenyl)-ethylamine 116% of the activity with 2-phenylethylamine Homo sapiens CoA + N-acetyl-2-(4-fluorophenyl)-ethylamine
-
 ?
2.3.1.87 acetyl-CoA + 2-(4-fluorophenyl)-ethylamine 60% of the activity with 2-phenylethylamine Ovis aries CoA + N-acetyl-2-(4-fluorophenyl)-ethylamine
-
 ?
2.3.1.87 acetyl-CoA + 2-(p-fluorophenyl)-ethylamine 56% of the activity with 2-phenylethylamine Homo sapiens CoA + N-acetyl-2-(p-fluorophenyl)-ethylamine
-
 ?
2.3.1.87 acetyl-CoA + 2-(p-fluorophenyl)-ethylamine as active as with 2-phenylethylamine Ovis aries CoA + N-acetyl-2-(p-fluorophenyl)-ethylamine
-
 ?
2.3.1.87 acetyl-CoA + 2-(p-nitrophenyl)-ethylamine 53% of the activity with 2-phenylethylamine Ovis aries CoA + N-acetyl-2-(p-nitrophenyl)-ethylamine
-
 ?
2.3.1.87 acetyl-CoA + 2-(p-nitrophenyl)-ethylamine 9% of the activity with 2-phenylethylamine Homo sapiens CoA + N-acetyl-2-(p-nitrophenyl)-ethylamine
-
 ?
2.3.1.87 acetyl-CoA + 2-(p-tolyl)-ethylamine 60% of the activity with 2-phenylethylamine Homo sapiens CoA + N-acetyl-2-(p-tolyl)-ethylamine
-
 ?
2.3.1.87 acetyl-CoA + 2-(p-tolyl)-ethylamine 87% of the activity with 2-phenylethylamine Ovis aries CoA + N-acetyl-2-(p-tolyl)-ethylamine
-
 ?
2.3.1.87 acetyl-CoA + 2-fluorophenylethylamine 69% of the activity with 2-phenylethylamine Homo sapiens CoA + ?
-
 ?
2.3.1.87 acetyl-CoA + 2-fluorophenylethylamine 52% of the activity with 2-phenylethylamine Ovis aries CoA + N-acetyl-2-fluorophenylethylamine
-
 ?
2.3.1.87 acetyl-CoA + 2-methoxyphenylethylamine 52% of the activity with 2-phenylethylamine Homo sapiens CoA + N-acetyl-2-methoxyphenylethylamine
-
 ?
2.3.1.87 acetyl-CoA + 2-methoxyphenylethylamine 97% of the activity with 2-phenylethylamine Ovis aries CoA + N-acetyl-2-methoxyphenylethylamine
-
 ?
2.3.1.87 acetyl-CoA + 2-phenylethylamine
-
 Ovis aries CoA + N-acetyl-2-phenylethylamine
-
 ?
2.3.1.87 acetyl-CoA + 2-phenylethylamine
-
 Rattus norvegicus CoA + N-acetyl-2-phenylethylamine
-
 ?
2.3.1.87 acetyl-CoA + 2-phenylethylamine
-
 Homo sapiens CoA + N-acetyl-2-phenylethylamine
-
 ?
2.3.1.87 acetyl-CoA + 3-hydroxy-4-methoxyphenethylamine 1% of the activity with 2-phenylethylamine Homo sapiens CoA + ?
-
 ?
2.3.1.87 acetyl-CoA + 3-hydroxy-4-methoxyphenethylamine 7.2% of the activity with 2-phenylethylamine Ovis aries CoA + N-acetyl-3-hydroxy-4-methoxyphenethylamine
-
 ?
2.3.1.87 acetyl-CoA + 3-hydroxyphenethylamine 24% of the activity with 2-phenylethylamine Homo sapiens CoA + ?
-
 ?
2.3.1.87 acetyl-CoA + 3-hydroxyphenethylamine 36% of the activity with 2-phenylethylamine Ovis aries CoA + N-acetyl-3-hydroxyphenethylamine
-
 ?
2.3.1.87 acetyl-CoA + 3-methoxy-2-phenylethylamine 23% of the activity with 2-phenylethylamine Homo sapiens CoA + ?
-
 ?
2.3.1.87 acetyl-CoA + 3-methoxy-2-phenylethylamine 55% of the activity with 2-phenylethylamine Ovis aries CoA + N-acetyl-3-methoxy-2-phenylethylamine
-
 ?
2.3.1.87 acetyl-CoA + 3-methoxytyramine 17% of the activity with 2-phenylethylamine Homo sapiens CoA + ?
-
 ?
2.3.1.87 acetyl-CoA + 3-methoxytyramine 73% of the activity with 2-phenylethylamine Ovis aries CoA + N-acetyl-3-methoxytyramine
-
 ?
2.3.1.87 acetyl-CoA + 4-(2-aminoethyl)-benzenesulfonamide 1% of the activity with 2-phenylethylamine Ovis aries CoA + ?
-
 ?
2.3.1.87 acetyl-CoA + 4-(2-aminoethyl)-benzenesulfonyl fluoride 7.4% of the activity with 2-phenylethylamine Ovis aries CoA + ?
-
 ?
2.3.1.87 acetyl-CoA + 4-(2-aminoethyl)-benzenesulfonyl fluoride 8% of the activity with 2-phenylethylamine Homo sapiens CoA + ?
-
 ?
2.3.1.87 acetyl-CoA + 4-methoxyphenylethylamine 10% of the activity with 2-phenylethylamine Ovis aries CoA + N-acetyl-4-methoxyphenylethylamine
-
 ?
2.3.1.87 acetyl-CoA + 4-methoxyphenylethylamine 11% of the activity with 2-phenylethylamine Homo sapiens CoA + N-acetyl-4-methoxyphenylethylamine
-
 ?
2.3.1.87 acetyl-CoA + 5-hydroxydopamine 4.4% of the activity with 2-phenylethylamine Ovis aries CoA + N-acetyl-5-hydroxydopamine
-
 ?
2.3.1.87 acetyl-CoA + 6-hydroxydopamine 51% of the activity with 2-phenylethylamine Homo sapiens CoA + ?
-
 ?
2.3.1.87 acetyl-CoA + 6-hydroxydopamine 1.5% of the activity with 2-phenylethylamine Ovis aries CoA + N-acetyl-6-hydroxydopamine
-
 ?
2.3.1.87 acetyl-CoA + serotonin
-
 Ovis aries CoA + N-acetylserotonin
-
 ?
2.3.1.87 acetyl-CoA + serotonin
-
 Rattus norvegicus CoA + N-acetylserotonin
-
 ?
2.3.1.87 acetyl-CoA + serotonin
-
 Homo sapiens CoA + N-acetylserotonin
-
 ?
2.3.1.87 acetyl-CoA + tyramine 10% of the activity with 2-phenylethylamine Ovis aries CoA + N-acetyltyramine
-
 ?
2.3.1.87 acetyl-CoA + tyramine 8% of the activity with 2-phenylethylamine Homo sapiens CoA + ?
-
 ?
2.3.1.87 additional information no activity with 2-(4-aminophenyl)-ethylamine, 3,4-(dibenzyloxy)phenethylamine or 2-(p-chlorophenoxy)-2-methylpropionic acid Ovis aries ?
-
 ?
2.3.1.87 additional information no activity with 2-(4-aminophenyl)-ethylamine, 3,4-(dibenzyloxy)phenethylamine, 5-hydroxydopamine, 4-(2-aminoethyl)-benzenesulfonamide or 2-(p-chlorophenoxy)-2-methylpropionic acid Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.87 hAANAT
-
 Homo sapiens
2.3.1.87 oAANAT
-
 Ovis aries
2.3.1.87 oAANAT
-
 Rattus norvegicus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.3.1.87 0.00018
-
 IC50: 0.00018 mM, GST-AANAT fusion protein Homo sapiens S 27481
2.3.1.87 0.00026
-
 IC50: 0.00026 mM, GST-AANAT fusion protein Ovis aries S 27244
2.3.1.87 0.00036
-
 IC50: 0.00036 mM, GST-AANAT fusion protein Rattus norvegicus S 27244
2.3.1.87 0.0004
-
 IC50: 0.0004 mM, GST-AANAT fusion protein Ovis aries S 27481
2.3.1.87 0.0004
-
 IC50: 0.0004 mM, GST-AANAT fusion protein Rattus norvegicus S 27481
2.3.1.87 0.00041
-
 IC50: 0.00041 mM, GST-AANAT fusion protein Homo sapiens acetyl-CoA-tryptamine
2.3.1.87 0.00062
-
 IC50: 0.00062 mM, GST-AANAT fusion protein Ovis aries acetyl-CoA-tryptamine
2.3.1.87 0.00072
-
 IC50: 0.00072 mM, GST-AANAT fusion protein Homo sapiens S 27244
2.3.1.87 0.00073
-
 IC50: 0.00073 mM, GST-AANAT fusion protein Ovis aries S 28036
2.3.1.87 0.0008
-
 IC50: 0.0008 mM, GST-AANAT fusion protein Rattus norvegicus acetyl-CoA-tryptamine
2.3.1.87 0.00128
-
 IC50: 0.00128 mM, GST-AANAT fusion protein Ovis aries bromoacetyltryptamine
2.3.1.87 0.0013
-
 IC50: 0.0013 mM, GST-AANAT fusion protein Ovis aries S 20251
2.3.1.87 0.00143
-
 IC50: 0.00143 mM, GST-AANAT fusion protein Homo sapiens bromoacetyltryptamine
2.3.1.87 0.0017
-
 IC50: 0.0017 mM, GST-AANAT fusion protein Rattus norvegicus S 28036
2.3.1.87 0.002
-
 IC50: 0.002 mM, GST-AANAT fusion protein Homo sapiens S 20251
2.3.1.87 0.0024
-
 IC50: 0.0024 mM, GST-AANAT fusion protein Rattus norvegicus S 20251
2.3.1.87 0.005
-
 IC50: 0.005 mM, GST-AANAT fusion protein Homo sapiens S 23823
2.3.1.87 0.0051
-
 IC50: 0.0051 mM, GST-AANAT fusion protein Rattus norvegicus bromoacetyltryptamine
2.3.1.87 0.0056
-
 IC50: 0.0056 mM,GST-AANAT fusion protein Homo sapiens S 28036
2.3.1.87 0.031
-
 IC50: 0.031 mM, GST-AANAT fusion protein Rattus norvegicus S 23823