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Literature summary extracted from

  • Kanugula, S.; Pauly, G.T.; Moschel, R.C.; Pegg, A.E.
    A bifunctional DNA repair protein from Ferroplasma acidarmanus exhibits O6-alkylguanine-DNA alkyltransferase and endonuclease V activities (2005), Proc. Natl. Acad. Sci. USA, 102, 3617-3622.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.63 expression in Escherichia coli Ferroplasma acidarmanus
3.1.21.7 expression in Escherichia coli GWR109 Ferroplasma acidarmanus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.63 DNA containing 4-O-methylthymine + [protein-L-cysteine Ferroplasma acidarmanus enzyme repairs O6-methylguanine lesions in DNA via alkyl transfer action DNA lacking 4-O-methylthymine + [protein]-S-methyl-L-cysteine
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 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.63 Ferroplasma acidarmanus
-
-
-
3.1.21.7 Ferroplasma acidarmanus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.63
-
 Ferroplasma acidarmanus
3.1.21.7 recombinant Ferroplasma acidarmanus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.63 DNA containing 4-O-methylthymine + [protein-L-cysteine
-
 Ferroplasma acidarmanus DNA lacking 4-O-methylthymine + [protein]-S-methyl-L-cysteine
-
 ?
2.1.1.63 DNA containing 4-O-methylthymine + [protein-L-cysteine enzyme repairs O6-methylguanine lesions in DNA via alkyl transfer action Ferroplasma acidarmanus DNA lacking 4-O-methylthymine + [protein]-S-methyl-L-cysteine
-
 ?
3.1.21.7 DNA + H2O the recombinant protein repais O6-methylguanine lesions in DNA via alkyl transfer action. The AGTendoV recombinant protein also cleaves DNA substrates that contain the deaminated bases uracil, hypoxanthine, or xanthine in a similar manner to Escherichia coli endonuclease V Ferroplasma acidarmanus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.63 AGTendoV
-
 Ferroplasma acidarmanus
2.1.1.63 O6-alkylguanine-DNA alkyltransferase
-
 Ferroplasma acidarmanus
3.1.21.7 AGTendoV bifunctional DNA repair protein from Ferroplasma acidarmanus exhibits O6-alkylguanine-DNA alkyltransferase and endonuclease V activities Ferroplasma acidarmanus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.63 46
-
-
 Ferroplasma acidarmanus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.1.1.63 37 70 about 50% of maximal activitya at 37°C and at 70°C Ferroplasma acidarmanus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.63 6.7
-
-
 Ferroplasma acidarmanus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.63 6 7.6 pH 6.0: about 80% of maximal activity, pH 7.6: about 40% of maximal activity Ferroplasma acidarmanus