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Literature summary extracted from

  • Liu, C.J.; Huhman, D.; Sumner, L.W.; Dixon, R.A.
    Regiospecific hydroxylation of isoflavones by cytochrome p450 81E enzymes from Medicago truncatula (2003), Plant J., 36, 471-484.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.90 expression in yeast strain WAT11 Medicago truncatula

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.88 0.05
-
 formononetin pH 8.0, 2 mM NADPH Medicago truncatula
1.14.14.88 0.11
-
 Biochanin A pH 8.0, 2 mM NADPH Medicago truncatula
1.14.14.90 0.051
-
 Biochanin A pH 8.0 Medicago truncatula
1.14.14.90 0.067
-
 formononetin pH 8.0 Medicago truncatula

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.88 endoplasmic reticulum membrane
-
 Medicago truncatula 5789
-
1.14.14.90 endoplasmic reticulum
-
 Medicago truncatula 5783
-

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.88 Medicago truncatula
-
-
-
1.14.14.90 Medicago truncatula Q6WNR0
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.88 leaf low mRNA levels Medicago truncatula
-
1.14.14.88 root MRNA is found after arbuscular mycorrhizal colonization Medicago truncatula
-
1.14.14.90 root
-
 Medicago truncatula
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.88 2'-hydroxyformononetin + [reduced NADPH-hemoprotein reductase] + O2 39% of activity with biochanin A Medicago truncatula 2',3'-dihydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.88 biochanin A + [reduced NADPH-hemoprotein reductase] + O2
-
 Medicago truncatula ?
-
 ?
1.14.14.88 formononetin + [reduced NADPH-hemoprotein reductase] + O2 50% of activity with biochanin A Medicago truncatula calycosin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.88 genistein + NADPH + O2 19% of activity with biochanin A Medicago truncatula ?
-
 ?
1.14.14.90 3'-hydroxyformononetin + [reduced NADPH-hemoprotein reductase] + O2 4.7% of the activity with biochanin A Medicago truncatula 2',3'-dihydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.90 biochanin A + [reduced NADPH-hemoprotein reductase] + O2
-
 Medicago truncatula 2'-hydroxybiochanin A + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.90 daidzein + [reduced NADPH-hemoprotein reductase] + O2 18.6% of the activity with biochanin A Medicago truncatula 2'-hydroxydaidzein + [oxidized NADPH-hemoprotein reductase] + H2O + low concentrations of 3'-hydroxydaidzein ?
1.14.14.90 formononetin + [reduced NADPH-hemoprotein reductase] + O2 37.2% of the activity with biochanin A Medicago truncatula 2'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.90 genistein + [reduced NADPH-hemoprotein reductase] + O2 10.2% of the activity with biochanin A Medicago truncatula 2'-hydroxygenistein + [oxidized NADPH-hemoprotein reductase] + H2O + low concentrations of 3'-hydroxygenistaien ?
1.14.14.90 additional information no activity with isoformononetin, prunetin, 6,7,4'-trihydroxyisoflavone, 3',4',7-trihydroxyisoflavone Medicago truncatula ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.88 MtCYP81E9
-
 Medicago truncatula
1.14.14.88 Mtl3'H
-
 Medicago truncatula
1.14.14.90 I2'H
-
 Medicago truncatula
1.14.14.90 MtCYP81E7
-
 Medicago truncatula

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.14.88 0.028
-
 formononetin pH 8.0, 2 mM NADPH Medicago truncatula
1.14.14.88 0.1
-
 Biochanin A pH 8.0, 2 mM NADPH Medicago truncatula
1.14.14.90 0.015
-
 formononetin pH 8.0 Medicago truncatula
1.14.14.90 0.033
-
 Biochanin A pH 8.0 Medicago truncatula

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.90 8
-
-
 Medicago truncatula

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.14.88 8
-
-
 Medicago truncatula

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.90 cytochrome P450
-
 Medicago truncatula