EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.14.90 | expression in yeast strain WAT11 | Medicago truncatula |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.14.88 | 0.05 | - |
formononetin | pH 8.0, 2 mM NADPH | Medicago truncatula | |
1.14.14.88 | 0.11 | - |
Biochanin A | pH 8.0, 2 mM NADPH | Medicago truncatula | |
1.14.14.90 | 0.051 | - |
Biochanin A | pH 8.0 | Medicago truncatula | |
1.14.14.90 | 0.067 | - |
formononetin | pH 8.0 | Medicago truncatula |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.14.14.88 | endoplasmic reticulum membrane | - |
Medicago truncatula | 5789 | - |
1.14.14.90 | endoplasmic reticulum | - |
Medicago truncatula | 5783 | - |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.88 | Medicago truncatula | - |
- |
- |
1.14.14.90 | Medicago truncatula | Q6WNR0 | - |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.14.88 | leaf | low mRNA levels | Medicago truncatula | - |
1.14.14.88 | root | MRNA is found after arbuscular mycorrhizal colonization | Medicago truncatula | - |
1.14.14.90 | root | - |
Medicago truncatula | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.88 | 2'-hydroxyformononetin + [reduced NADPH-hemoprotein reductase] + O2 | 39% of activity with biochanin A | Medicago truncatula | 2',3'-dihydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.88 | biochanin A + [reduced NADPH-hemoprotein reductase] + O2 | - |
Medicago truncatula | ? | - |
? | |
1.14.14.88 | formononetin + [reduced NADPH-hemoprotein reductase] + O2 | 50% of activity with biochanin A | Medicago truncatula | calycosin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.88 | genistein + NADPH + O2 | 19% of activity with biochanin A | Medicago truncatula | ? | - |
? | |
1.14.14.90 | 3'-hydroxyformononetin + [reduced NADPH-hemoprotein reductase] + O2 | 4.7% of the activity with biochanin A | Medicago truncatula | 2',3'-dihydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.90 | biochanin A + [reduced NADPH-hemoprotein reductase] + O2 | - |
Medicago truncatula | 2'-hydroxybiochanin A + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.90 | daidzein + [reduced NADPH-hemoprotein reductase] + O2 | 18.6% of the activity with biochanin A | Medicago truncatula | 2'-hydroxydaidzein + [oxidized NADPH-hemoprotein reductase] + H2O | + low concentrations of 3'-hydroxydaidzein | ? | |
1.14.14.90 | formononetin + [reduced NADPH-hemoprotein reductase] + O2 | 37.2% of the activity with biochanin A | Medicago truncatula | 2'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.90 | genistein + [reduced NADPH-hemoprotein reductase] + O2 | 10.2% of the activity with biochanin A | Medicago truncatula | 2'-hydroxygenistein + [oxidized NADPH-hemoprotein reductase] + H2O | + low concentrations of 3'-hydroxygenistaien | ? | |
1.14.14.90 | additional information | no activity with isoformononetin, prunetin, 6,7,4'-trihydroxyisoflavone, 3',4',7-trihydroxyisoflavone | Medicago truncatula | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.14.88 | MtCYP81E9 | - |
Medicago truncatula |
1.14.14.88 | Mtl3'H | - |
Medicago truncatula |
1.14.14.90 | I2'H | - |
Medicago truncatula |
1.14.14.90 | MtCYP81E7 | - |
Medicago truncatula |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.14.88 | 0.028 | - |
formononetin | pH 8.0, 2 mM NADPH | Medicago truncatula | |
1.14.14.88 | 0.1 | - |
Biochanin A | pH 8.0, 2 mM NADPH | Medicago truncatula | |
1.14.14.90 | 0.015 | - |
formononetin | pH 8.0 | Medicago truncatula | |
1.14.14.90 | 0.033 | - |
Biochanin A | pH 8.0 | Medicago truncatula |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.14.90 | 8 | - |
- |
Medicago truncatula |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.14.14.88 | 8 | - |
- |
Medicago truncatula |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.90 | cytochrome P450 | - |
Medicago truncatula |