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Literature summary extracted from

  • Madhavarao, C.N.; Chinopoulos, C.; Chandrasekaran, K.; Namboodiri, M.A.
    Characterization of the N-acetylaspartate biosynthetic enzyme from rat brain (2003), J. Neurochem., 86, 824-835.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.17 CHAPS 40% of activity at 12 mM, 10% of activity at 20 mM Rattus norvegicus
2.3.1.17 CoA IC50: 0.42 mM Rattus norvegicus
2.3.1.17 N-acetyl-L-aspartic acid IC50: 0.85 mM Rattus norvegicus
2.3.1.17 NaCl above 150 mM Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.17 0.058
-
 acetyl-CoA pH 7.1, 37°C Rattus norvegicus
2.3.1.17 0.58
-
 L-aspartate pH 7.1, 37°C Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.17 mitochondrion
-
 Rattus norvegicus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.17 670000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.17 acetyl-CoA + L-aspartate Rattus norvegicus decreases in enzyme activity occur in a number of neurological disorders, possible role in neuronal mitochondrial energy metabolism CoA + N-acetyl-L-aspartic acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.17 Rattus norvegicus
-
 male sprague-dawley rats
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.17 partial Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.17 brain
-
 Rattus norvegicus
-
2.3.1.17 neuron
-
 Rattus norvegicus
-
2.3.1.17 oligodendrocyte cultured Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.17 acetyl-CoA + L-aspartate
-
 Rattus norvegicus CoA + N-acetyl-L-aspartic acid
-
 ?
2.3.1.17 acetyl-CoA + L-aspartate decreases in enzyme activity occur in a number of neurological disorders, possible role in neuronal mitochondrial energy metabolism Rattus norvegicus CoA + N-acetyl-L-aspartic acid
-
 ?
2.3.1.17 additional information highly specific for L-aspartate with 3% or less active for L-glutamate, L-asparagine, L-glutamine, or aspartate-glutamate dipeptide Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.17 multimer at least 10 bands on SDS-PAGE indicating a large enzyme complex Rattus norvegicus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.3.1.17 0.42
-
 IC50: 0.42 mM Rattus norvegicus CoA
2.3.1.17 0.85
-
 IC50: 0.85 mM Rattus norvegicus N-acetyl-L-aspartic acid