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Literature summary extracted from
- Spectroscopic and kinetic characterization of active site mutants of Desulfovibrio fructosovoransNi-Fe hydrogenase (2003), J. Biol. Inorg. Chem., 8, 129-134.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.12.99.6 | - |
Solidesulfovibrio fructosivorans |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.12.99.6 | P498A | 100% of wild-type activity | Solidesulfovibrio fructosivorans |
| 1.12.99.6 | S499A | 100% of wild-type activity | Solidesulfovibrio fructosivorans |
| 1.12.99.6 | S499C | 75% of wild-type activity | Solidesulfovibrio fructosivorans |
| 1.12.99.6 | V78S | 75% of wild-type activity | Solidesulfovibrio fructosivorans |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.12.99.6 | Fe | contains Ni-Fe bimetallic center as active site | Solidesulfovibrio fructosivorans |  |
| 1.12.99.6 | Ni | contains Ni-Fe bimetallic center as active site | Solidesulfovibrio fructosivorans | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.12.99.6 | Solidesulfovibrio fructosivorans | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.12.99.6 | recombinant enzyme | Solidesulfovibrio fructosivorans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.12.99.6 | H2 + oxidized methyl viologen | - |
Solidesulfovibrio fructosivorans | reduced methyl viologen + H+ | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.12.99.6 | NiFe hydrogenase | - |
Solidesulfovibrio fructosivorans |
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Release 2023.1 (January 2023)
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