EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.1.142 | - |
Glycine max |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.142 | 24(28)-methylenecycloartanol | competitive with respect to S-adenosyl-L-methionine | Glycine max | |
2.1.1.142 | 25-Azacycloartenol | noncompetitive inhibitor versus cycloartenol, uncompetitive inhibitor versus S-adenosyl-L-methionine | Glycine max | |
2.1.1.142 | cyclolaudenol | dead end inhibitor analog, competitive versus cycloartenol, uncompetitive versus S-adenosyl-L-methionine | Glycine max | |
2.1.1.142 | S-adenosyl-L-homocysteine | noncompetitive with respect to S-adenosyl-L-methionine | Glycine max |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.142 | 0.02 | - |
24(28)-methylenelanosterol | - |
Glycine max | |
2.1.1.142 | 0.022 | - |
24(28)-methylenecycloartanol | - |
Glycine max | |
2.1.1.142 | 0.027 | - |
lanosterol | - |
Glycine max | |
2.1.1.142 | 0.03 | - |
Cycloartenol | - |
Glycine max | |
2.1.1.142 | 0.03 | - |
24(28)-methylenelophenol | - |
Glycine max | |
2.1.1.142 | 0.032 | - |
31-norcycloartenol | - |
Glycine max | |
2.1.1.142 | 0.035 | - |
Zymosterol | - |
Glycine max | |
2.1.1.142 | 0.043 | - |
parkeol | - |
Glycine max | |
2.1.1.142 | 0.045 | - |
24-dehydropollinstanol | - |
Glycine max |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.1.142 | 41500 | - |
4 * 41500, SDS-PAGE | Glycine max |
2.1.1.142 | 161000 | - |
gel filtration | Glycine max |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.142 | Glycine max | - |
recombinant enzyme | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.142 | recombinant enzyme | Glycine max |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.1.1.142 | additional information | - |
- |
Glycine max |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.142 | S-adenosyl-L-methionine + 24(28)-methylenecycloartanol | - |
Glycine max | S-adenosyl-L-homocysteine + ? | - |
? | |
2.1.1.142 | S-adenosyl-L-methionine + 24(28)-methylenelanosterol | - |
Glycine max | S-adenosyl-L-homocysteine + ? | - |
? | |
2.1.1.142 | S-adenosyl-L-methionine + 24(28)-methylenelophenol | - |
Glycine max | S-adenosyl-L-homocysteine + ? | - |
? | |
2.1.1.142 | S-adenosyl-L-methionine + 24-dehydropollinstanol | - |
Glycine max | S-adenosyl-L-homocysteine + ? | - |
? | |
2.1.1.142 | S-adenosyl-L-methionine + 31-norcycloartenol | - |
Glycine max | S-adenosyl-L-homocysteine + ? | - |
? | |
2.1.1.142 | S-adenosyl-L-methionine + cycloartenol | successive C-methylations of the DELTA24 bond occurs at the same active center. The two methylation steps can proceed by a change in chemical mechanism resulting from differences in sterol structure, concerted versus carbocation. The kinetic mechanism remains the same during the consecutive methylation of the DELTA24 bond | Glycine max | S-adenosyl-L-homocysteine + ? | - |
? | |
2.1.1.142 | S-adenosyl-L-methionine + lanosterol | - |
Glycine max | S-adenosyl-L-homocysteine + ? | - |
? | |
2.1.1.142 | S-adenosyl-L-methionine + parkeol | - |
Glycine max | S-adenosyl-L-homocysteine + ? | - |
? | |
2.1.1.142 | S-adenosyl-L-methionine + zymosterol | - |
Glycine max | S-adenosyl-L-homocysteine + ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.1.142 | tetramer | 4 * 41500, SDS-PAGE | Glycine max |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.142 | 0.00004 | - |
25-Azacycloartenol | inhibition versus cycloartenol | Glycine max | |
2.1.1.142 | 0.0001 | - |
25-Azacycloartenol | inhibition versus S-adenosyl-L-methionine | Glycine max | |
2.1.1.142 | 0.007 | - |
cyclolaudenol | inhibition versus cycloartenol | Glycine max | |
2.1.1.142 | 0.009 | - |
24(28)-methylene cycloartanol | inhibition versus S-adenosyl-L-methionine | Glycine max | |
2.1.1.142 | 0.009 | - |
cyclolaudenol | inhibition versus S-adenosyl-L-methionine | Glycine max | |
2.1.1.142 | 0.047 | - |
S-adenosyl-L-homocysteine | inhibition versus cycloartenol | Glycine max | |
2.1.1.142 | 0.056 | - |
S-adenosyl-L-homocysteine | inhibition versus S-adenosyl-L-methionine | Glycine max | |
2.1.1.142 | 0.171 | - |
24(28)-methylene cycloartanol | inhibition versus cycloartenol | Glycine max |