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Literature summary extracted from

  • Pinho, D.; Besson, S.; Brondino, C.D.; de Castro, B.; Moura, I.
    Copper-containing nitrite reductase from Pseudomonas chlororaphis DSM 50135 (2004), Eur. J. Biochem., 271, 2361-2369.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.2.1 copper 3.2 copper ions per trimer, most probably 3 type 1 and 3 type 2 copper, respectively Pseudomonas chlororaphis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.2.1 37700
-
 3 * 37700, SDS-PAGE Pseudomonas chlororaphis
1.7.2.1 107000
-
 gel filtration Pseudomonas chlororaphis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.2.1 nitrite + electron donor Pseudomonas chlororaphis
-
 NO + oxidized electron donor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.1 Pseudomonas chlororaphis
-
 DSM 50135
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.2.1 soluble fraction, DEAE-cellulose, Source 15Q, Sephadex 75 Pseudomonas chlororaphis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.2.1 0.33
-
 electron donor azurin Pseudomonas chlororaphis
1.7.2.1 130
-
 electron donor benzyl viologen Pseudomonas chlororaphis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.1 nitrite + electron donor
-
 Pseudomonas chlororaphis NO + oxidized electron donor
-
 ?
1.7.2.1 nitrite + reduced azurin azurin purified from Pseudomonas chlororaphis Pseudomonas chlororaphis NO + oxidized azurin
-
 ?
1.7.2.1 nitrite + reduced benzyl viologen
-
 Pseudomonas chlororaphis NO + oxidized benzyl viologen
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.2.1 trimer 3 * 37700, SDS-PAGE Pseudomonas chlororaphis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7.2.1 0.62
-
 nitrite pH 7.0, electron donor azurin Pseudomonas chlororaphis
1.7.2.1 243
-
 nitrite pH 7.0, electron donor benzyl viologen Pseudomonas chlororaphis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.7.2.1 Pseudomonas chlororaphis isoelectric focusing 6.2 5.4