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Literature summary extracted from

  • Fitzgerald, D.H.; Muirhead, K.M.; Botting, N.P.
    A comparative study on the inhibition of human and bacterial kynureninase by novel bicyclic kynurenine analogues (2001), Bioorg. Med. Chem., 9, 983-989.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.7.1.3 synthesis of inhibitors of kynureninase could prove to be useful in the development of the successful treatment regimen for neurological disorders such as septicemia, AIDS related dementia, Lyme disease, Huntington's and Alzheimer's disease Homo sapiens
3.7.1.3 synthesis of inhibitors of kynureninase could prove to be useful in the development of the successful treatment regimen for neurological disorders such as septicemia, AIDS related dementia, Lyme disease, Huntington's and Alzheimer's disease Pseudomonas fluorescens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.7.1.3 expression in Sf9 insect cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.7.1.3 2-amino-(4-oxo-4-naphthyl)-butyric acid competitive inhibition, reversible inhibition by dilution Homo sapiens
3.7.1.3 2-amino-(4-oxo-4-naphthyl)-butyric acid competitive inhibition, reversible inhibition by dilution Pseudomonas fluorescens
3.7.1.3 amino-(1-oxo-1,2,3,4-tetrahydro-2-naphthalenyl)-acetic acid competitive inhibition, reversible inhibition by dilution Homo sapiens
3.7.1.3 amino-(1-oxo-1,2,3,4-tetrahydro-2-naphthalenyl)-acetic acid competitive inhibition, reversible inhibition by dilution Pseudomonas fluorescens
3.7.1.3 amino-(1-oxo-2,3-dihydro-1H-inden-2-yl)-acetic acid competitive inhibition, reversible inhibition by dilution Homo sapiens
3.7.1.3 amino-(1-oxo-2,3-dihydro-1H-inden-2-yl)-acetic acid competitive inhibition, reversible inhibition by dilution Pseudomonas fluorescens
3.7.1.3 amino-(4-oxo-3,4-dihydro-2H-chromen-3-yl)-acetic acid competitive inhibition, reversible inhibition by dilution Homo sapiens
3.7.1.3 amino-(4-oxo-3,4-dihydro-2H-chromen-3-yl)-acetic acid competitive inhibition, reversible inhibition by dilution Pseudomonas fluorescens
3.7.1.3 beta-chloro-L-alanine irreversible inhibition Homo sapiens
3.7.1.3 beta-chloro-L-alanine irreversible inhibition Pseudomonas fluorescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.7.1.3 0.0442
-
 L-kynurenine at 37ºC, pH 7.5 Pseudomonas fluorescens
3.7.1.3 0.0442
-
 3-hydroxy-L-kynurenine at 37ºC, pH 7.5 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.7.1.3 3-hydroxy-L-kynurenine + H2O Homo sapiens biosynthesis of NAD+ 3-hydroxyanthranilate + L-alanine
-
 ?
3.7.1.3 3-hydroxy-L-kynurenine + H2O Pseudomonas fluorescens biosynthesis of NAD+ 3-hydroxyanthranilate + L-alanine
-
 ?
3.7.1.3 L-kynurenine + H2O Homo sapiens L-tryptophan metabolism anthranilate + L-alanine
-
 ?
3.7.1.3 L-kynurenine + H2O Pseudomonas fluorescens L-tryptophan metabolism anthranilate + L-alanine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.7.1.3 Homo sapiens
-
-
-
3.7.1.3 Pseudomonas fluorescens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.7.1.3 no modification
-
 Homo sapiens
3.7.1.3 no modification
-
 Pseudomonas fluorescens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.7.1.3 by protamine sulfate precipitation, ammonium sulfate precipitation, DEAE-Sepharose 6B column, Sephadex G-100 column, Mono-Q HR 5/5 column, 50-80fold Pseudomonas fluorescens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.7.1.3 culture medium
-
 Pseudomonas fluorescens
-
3.7.1.3 liver
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.7.1.3 0.008 0.015 pH 7.5, 37ºC Homo sapiens
3.7.1.3 1.2 2.2 pH 7.5, 37ºC Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.7.1.3 3-hydroxy-L-kynurenine + H2O
-
 Homo sapiens 3-hydroxyanthranilate + L-alanine
-
 ?
3.7.1.3 3-hydroxy-L-kynurenine + H2O
-
 Pseudomonas fluorescens 3-hydroxyanthranilate + L-alanine
-
 ?
3.7.1.3 3-hydroxy-L-kynurenine + H2O biosynthesis of NAD+ Homo sapiens 3-hydroxyanthranilate + L-alanine
-
 ?
3.7.1.3 3-hydroxy-L-kynurenine + H2O biosynthesis of NAD+ Pseudomonas fluorescens 3-hydroxyanthranilate + L-alanine
-
 ?
3.7.1.3 L-kynurenine + H2O
-
 Homo sapiens anthranilate + L-alanine
-
 ?
3.7.1.3 L-kynurenine + H2O
-
 Pseudomonas fluorescens anthranilate + L-alanine
-
 ?
3.7.1.3 L-kynurenine + H2O L-tryptophan metabolism Homo sapiens anthranilate + L-alanine
-
 ?
3.7.1.3 L-kynurenine + H2O L-tryptophan metabolism Pseudomonas fluorescens anthranilate + L-alanine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.7.1.3 L-kynurenine hydrolase
-
 Homo sapiens
3.7.1.3 L-kynurenine hydrolase
-
 Pseudomonas fluorescens

Cofactor

EC Number Cofactor Comment Organism Structure
3.7.1.3 pyridoxal 5'-phosphate
-
 Homo sapiens
3.7.1.3 pyridoxal 5'-phosphate
-
 Pseudomonas fluorescens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.7.1.3 0.005
-
 2-amino-(4-oxo-4-naphthyl)-butyric acid at 37ºC, pH 7.5 Pseudomonas fluorescens
3.7.1.3 0.022
-
 2-amino-(4-oxo-4-naphthyl)-butyric acid at 37ºC, pH 7.5 Homo sapiens
3.7.1.3 0.0349
-
 amino-(1-oxo-2,3-dihydro-1H-inden-2-yl)-acetic acid at 37ºC, pH 7.5 Pseudomonas fluorescens
3.7.1.3 0.045
-
 amino-(1-oxo-2,3-dihydro-1H-inden-2-yl)-acetic acid at 37ºC, pH 7.5 Homo sapiens
3.7.1.3 0.077
-
 amino-(4-oxo-3,4-dihydro-2H-chromen-3-yl)-acetic acid at 37ºC, pH 7.5 Homo sapiens
3.7.1.3 0.162
-
 amino-(4-oxo-3,4-dihydro-2H-chromen-3-yl)-acetic acid at 37ºC, pH 7.5 Pseudomonas fluorescens
3.7.1.3 0.17
-
 amino-(1-oxo-1,2,3,4-tetrahydro-2-naphthalenyl)-acetic acid at 37ºC, pH 7.5 Pseudomonas fluorescens
3.7.1.3 0.227
-
 amino-(1-oxo-1,2,3,4-tetrahydro-2-naphthalenyl)-acetic acid at 37ºC, pH 7.5 Homo sapiens