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Literature summary extracted from

  • Chen, X.; Wood, A.J.
    Purification and characterization of S-adenosyl-L-methionine:nicotinic acid-N-methyltransferase from leaves of Glycine max (2004), Biol. Plant., 48, 531-535.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.1.1.7 10 mM DTT and 20% glycerol stabilize the unstable enzyme during purification and storage Glycine max

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.7 Hg2+ at 1 mM, reversible by 10 mM DTT Glycine max
2.1.1.7 parahydroxymercuric benzoate at 0.1 mM, reversible by 10 mM DTT Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.7 0.0125
-
 nicotinate pH 7.0, 35°C Glycine max
2.1.1.7 0.031
-
 S-adenosyl-L-methionine pH 7.0, 35°C Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.7 41500
-
 2 * 41500, SDS-PAGE Glycine max
2.1.1.7 85000
-
 ge filtration Glycine max

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.7 S-adenosyl-L-methionine + nicotinate Glycine max key enzyme in regulating the metabolic flux through the nicotinamide pathway S-adenosyl-L-homocysteine + N-methylnicotinate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.7 Glycine max
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.7 2650fold from leaves, to homogeneity, by ammonium sulfate fractionation, DEAE ion exchange chromatography, adenosine affinity chromatography, hydroxyl apatite chromatography, and gel filtration Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.7 leaf
-
 Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.7 0.0031
-
 purified enzyme Glycine max

Storage Stability

EC Number Storage Stability Organism
2.1.1.7 -20°C, purified enzyme, 20 mM Tris-HCl, pH 8.0, 20 mM DTT, 20% glycerol, stable for 14 days Glycine max
2.1.1.7 4°C, purified enzyme, 20 mM Tris-HCl, pH 8.0, 20 mM DTT, 20% glycerol, stable for 3 days Glycine max
2.1.1.7 4°C, purified enzyme, 20 mM Tris-HCl, pH 8.0, 20% glycerol, complete loss of activity within 24 h Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.7 S-adenosyl-L-methionine + nicotinate key enzyme in regulating the metabolic flux through the nicotinamide pathway Glycine max S-adenosyl-L-homocysteine + N-methylnicotinate
-
 ?
2.1.1.7 S-adenosyl-L-methionine + nicotinate specific for nicotinic acid Glycine max S-adenosyl-L-homocysteine + N-methylnicotinate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.7 dimer 2 * 41500, SDS-PAGE Glycine max

Synonyms

EC Number Synonyms Comment Organism
2.1.1.7 NNMT
-
 Glycine max
2.1.1.7 S-adenosyl-L-methionine:nicotinic acid-N-methyltransferase
-
 Glycine max

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.7 40 45
-
 Glycine max

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.1.1.7 40 50 50% remaining activity at 50°C Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.7 6.5
-
-
 Glycine max

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.7 4.3 8.8 half-maximal activity at pH 5.4 and pH 7.5 Glycine max