EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.12.99.6 | Cd2+ | reversible inhibition | Lamprobacter modestohalophilus | |
1.12.99.6 | CO | noncompetitive vs. oxidized methyl viologen, competitive vs. H2 | Lamprobacter modestohalophilus | |
1.12.99.6 | Mg2+ | reversible inhibition | Lamprobacter modestohalophilus | |
1.12.99.6 | Ni2+ | competitive vs. Oxidized methyl viologen | Lamprobacter modestohalophilus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.12.99.6 | soluble | - |
Lamprobacter modestohalophilus | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.12.99.6 | 30000 | - |
1 * 64000 + 1 * 30000, SDS-PAGE | Lamprobacter modestohalophilus |
1.12.99.6 | 64000 | - |
1 * 64000 + 1 * 30000, SDS-PAGE | Lamprobacter modestohalophilus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.12.99.6 | Lamprobacter modestohalophilus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.12.99.6 | - |
Lamprobacter modestohalophilus |
EC Number | Storage Stability | Organism |
---|---|---|
1.12.99.6 | 20°C, 50 mM Tris-HCl, pH 7.0, 0.02% NaN3, storage under air, 50 d, approx. 80% loss of activity | Lamprobacter modestohalophilus |
1.12.99.6 | 20°C, 50 mM Tris-HCl, pH 7.0, 0.02% NaN3, storage under argon, 50 d, approx. 60% loss of activity | Lamprobacter modestohalophilus |
1.12.99.6 | 20°C, 50 mM Tris-HCl, pH 7.0, 0.02% NaN3, storage under H2, 50 d, approx. 70% loss of activity | Lamprobacter modestohalophilus |
1.12.99.6 | 4°C, 50 mM Tris-HCl, pH 7.0, 0.02% NaN3, storage under air, 60 d, approx. 40% loss of activity | Lamprobacter modestohalophilus |
1.12.99.6 | 4°C, 50 mM Tris-HCl, pH 7.0, 0.02% NaN3, storage under argon, 60 d, approx. 30% loss of activity | Lamprobacter modestohalophilus |
1.12.99.6 | 4°C, 50 mM Tris-HCl, pH 7.0, 0.02% NaN3, storage under H2, 60 d, approx. 50% loss of activity | Lamprobacter modestohalophilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.12.99.6 | H2 + oxidized benzyl viologen | - |
Lamprobacter modestohalophilus | reduced benzyl viologen + H+ | - |
r | |
1.12.99.6 | H2 + oxidized methyl viologen | - |
Lamprobacter modestohalophilus | reduced methyl viologen + H+ | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.12.99.6 | dimer | 1 * 64000 + 1 * 30000, SDS-PAGE | Lamprobacter modestohalophilus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.12.99.6 | 85 | - |
- |
Lamprobacter modestohalophilus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.12.99.6 | 20 | 95 | almost no activity at 20°C, approx. 20% of maximal activity at 95°C | Lamprobacter modestohalophilus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.12.99.6 | 70 | - |
no loss of activity after 1.5 min at pH 7.0 | Lamprobacter modestohalophilus |
1.12.99.6 | 85 | - |
approx. 50% loss of activity after 1.5 min at pH 7.0 | Lamprobacter modestohalophilus |
1.12.99.6 | 100 | - |
almost complete loss of activity after 1.5 min at pH 7.0 | Lamprobacter modestohalophilus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.12.99.6 | 4 | - |
H2 evolution | Lamprobacter modestohalophilus |
1.12.99.6 | 8.5 | 9.5 | H2 oxidation | Lamprobacter modestohalophilus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.12.99.6 | 0.007 | - |
CO | 30°C, pH 7.0 | Lamprobacter modestohalophilus | |
1.12.99.6 | 0.16 | - |
Ni2+ | 30°C, pH 9.0 | Lamprobacter modestohalophilus | |
1.12.99.6 | 1.35 | - |
Ni2+ | 30°C, pH 7.0 | Lamprobacter modestohalophilus |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.12.99.6 | Lamprobacter modestohalophilus | isoelectric focusing | - |
4.5 |