Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Cunane, L.M.; Chen, Z.W.; McIntire, W.S.; Mathews, F.S.
    p-Cresol methylhydroxylase: alteration of the structure of the flavoprotein subunit upon its binding to the cytochrome subunit (2005), Biochemistry, 44, 2963-2973.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.17.9.1 expressed in Escherichia coli Pseudomonas putida

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.17.9.1 native enzyme and alpha subunit, sitting drop vapor diffusion method Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.9.1 9200
-
 alpha2beta2, 2 * 58700 + 2 * 9200, c-type cytochrome subunit PchC (beta) and two flavoprotein subunits PchF (alpha) Pseudomonas putida
1.17.9.1 58700
-
 alpha2beta2, 2 * 58700 + 2 * 9200, c-type cytochrome subunit PchC (beta) and two flavoprotein subunits PchF (alpha) Pseudomonas putida
1.17.9.1 136000
-
-
 Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.9.1 4-cresol + acceptor + H2O Pseudomonas putida via intermediate 4-hydroxybenzylalcohol, degradation of toxic 4-cresol 4-hydroxybenzaldehyde + reduced acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.9.1 Pseudomonas putida P09788
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.9.1 recombinant enzyme Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.9.1 4-cresol + acceptor + H2O via intermediate 4-hydroxybenzylalcohol Pseudomonas putida 4-hydroxybenzaldehyde + reduced acceptor
-
 ?
1.17.9.1 4-cresol + acceptor + H2O via intermediate 4-hydroxybenzylalcohol, degradation of toxic 4-cresol Pseudomonas putida 4-hydroxybenzaldehyde + reduced acceptor
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.9.1 tetramer alpha2beta2, 2 * 58700 + 2 * 9200, c-type cytochrome subunit PchC (beta) and two flavoprotein subunits PchF (alpha) Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.9.1 FAD
-
 Pseudomonas putida