Literature summary extracted from
Veitch, N.J.; Maugeri, D.A.; Cazzulo, J.J.; Lindqvist, Y.; Barrett, M.P.
Transketolase from Leishmania mexicana has a dual subcellular localization (2004), Biochem. J., 382, 759-767.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.2.1.1 |
- |
Leishmania mexicana |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
2.2.1.1 |
- |
Leishmania mexicana |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
2.2.1.1 |
D-ribose-5-phosphate |
substrate inhibition above 50 mM |
Leishmania mexicana |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
2.2.1.1 |
2.75 |
- |
D-ribose-5-phosphate |
pH 7.5 |
Leishmania mexicana |
|
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
2.2.1.1 |
cytosol |
predominantly cytosolic |
Leishmania mexicana |
5829 |
- |
2.2.1.1 |
glycosome |
significant part of total activity |
Leishmania mexicana |
20015 |
- |
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
2.2.1.1 |
70000 |
- |
crystallization data, 2 * 70000, SDS-PAGE, 2 * 71800, deduced from gene sequence |
Leishmania mexicana |
2.2.1.1 |
71800 |
- |
crystallization data, 2 * 70000, SDS-PAGE, 2 * 71800, deduced from gene sequence |
Leishmania mexicana |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.2.1.1 |
Leishmania mexicana |
Q8MPM3 |
expression in Escherichia coli |
- |
Posttranslational Modification
EC Number |
Posttranslational Modification |
Comment |
Organism |
---|
2.2.1.1 |
proteolytic modification |
type-1 peroxisome targeting signal |
Leishmania mexicana |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
2.2.1.1 |
1.7 |
- |
pH 7.5 |
Leishmania mexicana |
Storage Stability
EC Number |
Storage Stability |
Organism |
---|
2.2.1.1 |
-80°C, without glycerol, stable |
Leishmania mexicana |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.2.1.1 |
D-xylulose 5-phosphate + D-ribose 5-phosphate |
- |
Leishmania mexicana |
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
2.2.1.1 |
dimer |
crystallization data, 2 * 70000, SDS-PAGE, 2 * 71800, deduced from gene sequence |
Leishmania mexicana |
Turnover Number [1/s]
EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Comment |
Organism |
Structure |
---|
2.2.1.1 |
8.4 |
- |
D-ribose-5-phosphate |
pH 7.5 |
Leishmania mexicana |
|