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Literature summary extracted from

  • Guerin, M.E.; Buschiazzo, A.; Kordulakova, J.; Jackson, M.; Alzari, P.M.
    Crystallization and preliminary crystallographic analysis of PimA, an essential mannosyltransferase from Mycobacterium smegmatis (2005), Acta Crystallogr. Sect. F, 61, 518-520.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.345 expression in Escherichia coli Mycolicibacterium smegmatis
2.4.1.345 expression in Escherichia coli BL21(DE3)pLysS cells transformed wth pET-pimA Mycolicibacterium smegmatis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.1.345 in the presence of GDP and myo-inositol, to 2.4 A resolution, orthorhombic space group P212121, one molecule per asymmetric unit Mycolicibacterium smegmatis
2.4.1.345 sitting-drop vapour-diffusion method. The crystals belong to space group P2(1)2(1)2(1) with unit-cell parameters a = 37.2, b = 72.4, c = 138.2 A and diffract to 2.4 A resolution Mycolicibacterium smegmatis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.345 43300
-
-
 Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.345 additional information Mycolicibacterium smegmatis essential enzyme for mycobacterial growth ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.345 Mycolicibacterium smegmatis
-
-
-
2.4.1.345 Mycolicibacterium smegmatis A0QWG6
-
-
2.4.1.345 Mycolicibacterium smegmatis ATCC 700084 A0QWG6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.345
-
 Mycolicibacterium smegmatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.345 additional information essential enzyme for mycobacterial growth Mycolicibacterium smegmatis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.345 ? x * 43300, SDS-PAGE Mycolicibacterium smegmatis

Synonyms

EC Number Synonyms Comment Organism
2.4.1.345 phosphatidylinositol mannosyltransferase
-
 Mycolicibacterium smegmatis
2.4.1.345 PimA
-
 Mycolicibacterium smegmatis