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Literature summary extracted from

  • Iyer, P.P.; Lawrence, S.H.; Yennawar, H.P.; Ferry, J.G.
    Expression, purification, crystallization and preliminary X-ray analysis of phosphotransacetylase from Methanosarcina thermophila (2003), Acta Crystallogr. Sect. D, 59, 1517-1520.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.8 expressed in soluble form in Escherichia coli BL21-DE3 Methanosarcina thermophila

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.8 hanging drop vapor diffusion method Methanosarcina thermophila

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.8 71300
-
 calculated from hydrodynamic radius obtained from dynamic light scattering Methanosarcina thermophila

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.8 acetyl-CoA + phosphate Methanosarcina thermophila
-
 CoA + acetyl phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.8 Methanosarcina thermophila
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.8 recombinant enzyme from Escherichia coli Methanosarcina thermophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.8 acetyl-CoA + phosphate
-
 Methanosarcina thermophila CoA + acetyl phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.3.1.8 dimer in solution, dynamic light scattering Methanosarcina thermophila

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.8 4500
-
 acetyl-CoA
-
 Methanosarcina thermophila