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Literature summary extracted from

  • Schroeder, G.; Wehinger, E.; Lukacin, R.; Wellmann, F.; Seefelder, W.; Schwab, W.; Schroeder, J.
    Flavonoid methylation: a novel 4'-O-methyltransferase from Catharanthus roseus, and evidence that partially methylated flavanones are substrates of four different flavonoid dioxygenases (2004), Phytochemistry, 65, 1085-1094.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.155 CrOMT6 gene, expression in Escherichia coli, gene structure Catharanthus roseus
2.1.1.231 gene CrOMT6, DNA and amino acid sequence determination and analysis, qualitative RT-PCR expression analysis, sequence comparison and phylogenetic tree, expression of His-tagged OMT6 in Escherichia coli Catharanthus roseus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.155 soluble recombinant enzyme Catharanthus roseus
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.231 S-adenosyl-L-methionine + 3'-O-methyleriodictyol Catharanthus roseus
-
 S-adenosyl-L-homocysteine + 3',4'-O-dimethyleriodictyol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.20.5 Petroselinum crispum
-
 expressed in yeast
-
2.1.1.155 Catharanthus roseus
-
 line CP3a
-
2.1.1.231 Catharanthus roseus Q6VCW3 gene CrOMT6
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.155 recombinant enzyme Catharanthus roseus
2.1.1.231 recombinant His-tagged OMT6 in Escherichia coli by nickel affinity chromatgraphy Catharanthus roseus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.155 cell suspension culture
-
 Catharanthus roseus
-
2.1.1.231 cell suspension culture
-
 Catharanthus roseus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.20.5 eriodictyol + 2-oxoglutarate + O2 24% of the activity with naringenin Petroselinum crispum ?
-
 ?
1.14.20.5 hesperetin + 2-oxoglutarate + O2 26% of the activity with naringenin Petroselinum crispum 3,5-dihydroxy-2-(3-hydroxy-4-methoxyphenyl)-chromen-4-one + succinate + CO2 + H2O
-
 ?
1.14.20.5 homoeriodictyol + 2-oxoglutarate + O2 97% of the activity with naringenin Petroselinum crispum 5,7,4'-trihydroxy-3'-methoxyflavone + succinate + CO2 + H2O
-
 ?
1.14.20.5 additional information no activity with 5,7-dihydroxy-3',4',5'-O-trimethyl-flavanone,7-O-methyl-pinocembrin, 5-O-methyl-pinocembrin, 5,7-O-dimethyl-pinocembrin Petroselinum crispum ?
-
 ?
1.14.20.5 naringenin + 2-oxoglutarate + O2
-
 Petroselinum crispum apigenin + succinate + CO2 + H2O
-
 ?
1.14.20.5 pinocembrin + 2-oxoglutarate + O2 24% of the activity with naringenin Petroselinum crispum chrysin + succinate + CO2 + H2O
-
 ?
2.1.1.155 additional information not: naringenin, pentahydroxyflavanone, hesperetin, myricetin, 7,3’-O-dimethylquercetin, 7-O-methylquercetin, syringetin, apigenin, luteolin, tricetin, velutin, dihydrokaempferol, dihydroquercetin, dihydromyricetin, 3’-O-methyl-dihydroquercetin Catharanthus roseus ?
-
 ?
2.1.1.155 S-adenosyl-L-methionine + a flavonoid 4’-OMT methylates the position 4’ in the B-ring of flavonoids, uses flavonoids methylated in the 3’-position of the B-ring to synthesize a 3’,4’-methylated product, a methylated flavanone, homoeriodictyol, is the best substrate Catharanthus roseus S-adenosyl-L-homocysteine + a 4'-methoxyflavonoid
-
 ?
2.1.1.155 S-adenosyl-L-methionine + chrysoeriol good substrate Catharanthus roseus S-adenosyl-L-homocysteine + 4'-methoxychrysoeriol
-
 ?
2.1.1.155 S-adenosyl-L-methionine + eriodictyol lower activity than with homoeriodictyol, isorhamnetin or chrysoeriol Catharanthus roseus S-adenosyl-L-homocysteine + 4'-methoxyeriodictyol
-
 ?
2.1.1.155 S-adenosyl-L-methionine + homoeriodictyol best substrate Catharanthus roseus S-adenosyl-L-homocysteine + 4'-methoxyhomoeriodictyol
-
 ?
2.1.1.155 S-adenosyl-L-methionine + isorhamnetin good substrate Catharanthus roseus S-adenosyl-L-homocysteine + 4'-methoxyisorhamnetin
-
 ?
2.1.1.155 S-adenosyl-L-methionine + kaempferol lower activity than with homoeriodictyol, isorhamnetin or chrysoeriol Catharanthus roseus S-adenosyl-L-homocysteine + kaempferide
-
 ?
2.1.1.155 S-adenosyl-L-methionine + quercetin lower activity than with homoeriodictyol, isorhamnetin or chrysoeriol Catharanthus roseus S-adenosyl-L-homocysteine + 4'-methoxyquercetin
-
 ?
2.1.1.231 additional information the only substrates found for CrOMT6 are 3'-O-methyl-eriodictyol (homoeriodictyol) and the corresponding flavones and flavonols, substrate specificty, overview. No or poor activity with naringenin, pentahydroxyflavanone, hesperetin, myricetin, 7,3'-O-dimethylquercetin, 7-O-methylquercetin, syringetin, apigenin, luteolin, tricetin, velutin, dihydrokaempferol, dihydroquercetin, dihydromyricetin, and 3'-O-methyl-dihydroquercetin, and also no activity with ferulic acid, coniferyl alcohol, vanillic acid vanillin, eugenol, isoeugenol, and guaicol. Not only the B-ring configuration, but also the size and shape of the A-ring are critical parts of the substrate specificity Catharanthus roseus ?
-
 ?
2.1.1.231 S-adenosyl-L-methionine + 3'-O-methyleriodictyol
-
 Catharanthus roseus S-adenosyl-L-homocysteine + 3',4'-O-dimethyleriodictyol
-
 ?
2.1.1.231 S-adenosyl-L-methionine + 3'-O-methyleriodictyol
-
 Catharanthus roseus S-adenosyl-L-homocysteine + 3',4'-O-dimethyleriodictyol product identification by mass spectrometry ?
2.1.1.231 S-adenosyl-L-methionine + chrysoeriol
-
 Catharanthus roseus S-adenosyl-L-homocysteine + 4'-O-methylchrysoeriol
-
 ?
2.1.1.231 S-adenosyl-L-methionine + eriodictyol
-
 Catharanthus roseus S-adenosyl-L-homocysteine + 4'-O-methyleriodictyol
-
 ?
2.1.1.231 S-adenosyl-L-methionine + isorhamnetin
-
 Catharanthus roseus S-adenosyl-L-homocysteine + 4'-O-methylisorhamnetin
-
 ?
2.1.1.231 S-adenosyl-L-methionine + kaempferol
-
 Catharanthus roseus S-adenosyl-L-homocysteine + 4'-O-methylkaempferol
-
 ?
2.1.1.231 S-adenosyl-L-methionine + quercetin
-
 Catharanthus roseus S-adenosyl-L-homocysteine + 4'-O-methylquercetin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.20.5 FNS
-
 Petroselinum crispum
2.1.1.155 4'OMT
-
 Catharanthus roseus
2.1.1.155 CrOMT6
-
 Catharanthus roseus
2.1.1.231 4'-O-methyltransferase
-
 Catharanthus roseus
2.1.1.231 4'OMT
-
 Catharanthus roseus
2.1.1.231 B-ring 4'OMT
-
 Catharanthus roseus
2.1.1.231 flavonoid 4'-O-methyltransferase UniProt Catharanthus roseus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.155 7.5
-
 assay at Catharanthus roseus
2.1.1.231 7.5
-
 assay at Catharanthus roseus

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.231 S-adenosyl-L-methionine
-
 Catharanthus roseus