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Literature summary extracted from

  • Till, B.J.; Burtner, C.; Comai, L.; Henikoff, S.
    Mismatch cleavage by single-strand specific nucleases (2004), Nucleic Acids Res., 32, 2632-2641.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.30.1
-
 Apium graveolens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.30.1 Mg2+ 10 mM, cleavage of single base pair mismatches Vigna radiata
3.1.30.1 Mg2+ 10 mM, cleavage of single base pair mismatches Aspergillus oryzae
3.1.30.1 Mg2+ 10 mM, targeting induced local lesions in genomes mismatch cleavage protocol Apium graveolens
3.1.30.1 Zn2+ 0.2 mM, cleavage of single base pair mismatches Vigna radiata
3.1.30.1 Zn2+ 2 mM, cleavage of single base pair mismatches Aspergillus oryzae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.30.1 heteroduplex DNA + H2O Vigna radiata cleavage of single base pair mismatches in heteroduplex DNA nicked DNA
-
 ?
3.1.30.1 heteroduplex DNA + H2O Aspergillus oryzae cleavage of single base pair mismatches in heteroduplex DNA nicked DNA
-
 ?
3.1.30.1 heteroduplex DNA + H2O Apium graveolens cleavage of single base pair mismatches in heteroduplex DNA nicked DNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.30.1 Apium graveolens Q9LL59 celery
-
3.1.30.1 Aspergillus oryzae
-
-
-
3.1.30.1 Vigna radiata
-
 mung bean
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.30.1
-
 Vigna radiata
3.1.30.1
-
 Aspergillus oryzae
3.1.30.1
-
 Apium graveolens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.30.1 sprout
-
 Vigna radiata
-
3.1.30.1 sprout
-
 Aspergillus oryzae
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.30.1 heteroduplex DNA + H2O cleavage of single base pair mismatches in heteroduplex DNA Vigna radiata nicked DNA
-
 ?
3.1.30.1 heteroduplex DNA + H2O cleavage of single base pair mismatches in heteroduplex DNA Aspergillus oryzae nicked DNA
-
 ?
3.1.30.1 heteroduplex DNA + H2O cleavage of single base pair mismatches in heteroduplex DNA Apium graveolens nicked DNA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.30.1 CEL I
-
 Apium graveolens
3.1.30.1 S1
-
 Aspergillus oryzae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.30.1 45
-
 cleavage of single base pair mismatches Aspergillus oryzae
3.1.30.1 45
-
 targeting induced local lesions in genomes mismatch cleavage protocol Apium graveolens
3.1.30.1 60
-
 cleavage of single base pair mismatches Vigna radiata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.30.1 5.5
-
 cleavage of single base pair mismatches Aspergillus oryzae
3.1.30.1 6.5
-
 cleavage of single base pair mismatches Vigna radiata
3.1.30.1 7.5
-
 targeting induced local lesions in genomes mismatch cleavage protocol Apium graveolens