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Literature summary extracted from

  • Locatelli, G.A.; Gosselin, G.; Spadari, S.; Maga, G.
    Hepatitis C virus NS3 NTPase/helicase:different stereoselectivity in nucleoside triphosphate utilisation suggests that NTPase and helicase activities are coupled by a nucleotide-dependent rate limiting step (2001), J. Mol. Biol., 313, 683-694.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.6.1.15 medicine considered attractive target for anti-hepatitis C virus chemoterapy Hepacivirus C

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.15 2',3'-didehydro-thymidine triphosphate competitive to ATP Hepacivirus C
3.6.1.15 2',3'-dideoxy-5-fluorocytidine triphosphate competitive to ATP Hepacivirus C
3.6.1.15 3'-azido-2',3'-dideoxythymidine triphosphate competitive to ATP Hepacivirus C
3.6.1.15 ATP
-
 Hepacivirus C
3.6.1.15 dATP competitive to ATP Hepacivirus C
3.6.1.15 dCTP competitive to ATP Hepacivirus C
3.6.1.15 ddCTP competitive to ATP Hepacivirus C
3.6.1.15 ddTTP competitive to ATP Hepacivirus C
3.6.1.15 dGTP competitive to ATP Hepacivirus C
3.6.1.15 dTTP competitive to ATP Hepacivirus C
3.6.1.15 L-dATP competitive to ATP Hepacivirus C
3.6.1.15 L-dCTP competitive to ATP Hepacivirus C
3.6.1.15 L-ddCTP competitive to ATP Hepacivirus C
3.6.1.15 L-dTTP competitive to ATP Hepacivirus C

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.15 0.45
-
 ATP pH 7.5, 25°C, NTP hydrolysis-dependent helicase activity Hepacivirus C
3.6.1.15 0.54
-
 ATP pH 7.5, 25°C, NTPase activity Hepacivirus C
3.6.1.15 0.8
-
 2',3'-didehydro-thymidine triphosphate pH 7.5, 25°C, NTP hydrolysis-dependent helicase activity Hepacivirus C
3.6.1.15 0.8
-
 L-dATP pH 7.5, 25°C, NTP hydrolysis-dependent helicase activity Hepacivirus C
3.6.1.15 0.9
-
 ddTTP pH 7.5, 25°C, NTP hydrolysis-dependent helicase activity Hepacivirus C
3.6.1.15 2.3
-
 3'-azido-2',3'-dideoxythymidine triphosphate pH 7.5, 25°C, NTP hydrolysis-dependent helicase activity Hepacivirus C
3.6.1.15 2.4
-
 dTTP pH 7.5, 25°C, NTP hydrolysis-dependent helicase activity Hepacivirus C
3.6.1.15 3.8
-
 ddCTP pH 7.5, 25°C, NTP hydrolysis-dependent helicase activity Hepacivirus C
3.6.1.15 4
-
 dCTP pH 7.5, 25°C, NTP hydrolysis-dependent helicase activity Hepacivirus C
3.6.1.15 6.9
-
 dATP pH 7.5, 25°C, NTP hydrolysis-dependent helicase activity Hepacivirus C
3.6.1.15 9.9
-
 L-dCTP pH 7.5, 25°C, NTP hydrolysis-dependent helicase activity Hepacivirus C
3.6.1.15 28
-
 L-dTTP pH 7.5, 25°C, NTP hydrolysis-dependent helicase activity Hepacivirus C

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.15 additional information Hepacivirus C multifunctional protein, possessing protease, NTPase and helicase activities. These activities are essential for the virus life cycle ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.15 Hepacivirus C
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.15 2',3'-didehydro-thymidine triphosphate + H2O
-
 Hepacivirus C 2',3'-didehydro-thymidine diphosphate + phosphate
-
 ?
3.6.1.15 3'-azido-2',3'-dideoxythymidine triphosphate + H2O
-
 Hepacivirus C 3'-azido-2',3'-dideoxythymidine diphosphate + phosphate
-
 ?
3.6.1.15 ATP + H2O
-
 Hepacivirus C ADP + phosphate
-
 ?
3.6.1.15 dATP + H2O
-
 Hepacivirus C dADP + phosphate
-
 ?
3.6.1.15 dCTP + H2O
-
 Hepacivirus C dCDP + phosphate
-
 ?
3.6.1.15 ddCTP + H2O
-
 Hepacivirus C ddCDP + phosphate
-
 ?
3.6.1.15 ddTTP + H2O
-
 Hepacivirus C ddTDP + phosphate
-
 ?
3.6.1.15 dTTP + H2O
-
 Hepacivirus C TDP + phosphate
-
 ?
3.6.1.15 L-dATP + H2O
-
 Hepacivirus C L-dADP + phosphate
-
 ?
3.6.1.15 L-dCTP + H2O
-
 Hepacivirus C CDP + phosphate
-
 ?
3.6.1.15 L-dTTP + H2O
-
 Hepacivirus C L-dTDP + phosphate
-
 ?
3.6.1.15 additional information multifunctional protein, possessing protease, NTPase and helicase activities. These activities are essential for the virus life cycle Hepacivirus C ?
-
 ?
3.6.1.15 additional information multifunctional protein, possessing protease, NTPase and helicase activities. Different stereoselectivity in nucleoside triphosphate utilisation suggests that NTPase and helicase activities are coupled by a nucleotide-dependent rate limiting step Hepacivirus C ?
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.6.1.15 0.54
-
 ATP pH 7.5, 25°C, inhibition of ATPase activity Hepacivirus C
3.6.1.15 0.6
-
 dATP pH 7.5, 25°C, inhibition of ATPase activity Hepacivirus C
3.6.1.15 0.6
-
 2',3'-didehydro-thymidine triphosphate pH 7.5, 25°C, inhibition of ATPase activity Hepacivirus C
3.6.1.15 1.2
-
 ddTTP pH 7.5, 25°C, inhibition of ATPase activity Hepacivirus C
3.6.1.15 1.2
-
 L-dATP pH 7.5, 25°C, inhibition of ATPase activity Hepacivirus C
3.6.1.15 1.3
-
 L-dTTP pH 7.5, 25°C, inhibition of ATPase activity Hepacivirus C
3.6.1.15 1.5
-
 L-dCTP pH 7.5, 25°C, inhibition of ATPase activity Hepacivirus C
3.6.1.15 2.1
-
 3'-azido-2',3'-dideoxythymidine triphosphate pH 7.5, 25°C, inhibition of ATPase activity Hepacivirus C
3.6.1.15 2.3
-
 L-ddCTP pH 7.5, 25°C, inhibition of ATPase activity Hepacivirus C
3.6.1.15 2.5
-
 2',3'-dideoxy-5-fluorocytidine triphosphate pH 7.5, 25°C, inhibition of ATPase activity Hepacivirus C
3.6.1.15 2.8
-
 dTTP pH 7.5, 25°C, inhibition of ATPase activity Hepacivirus C
3.6.1.15 3
-
 dCTP pH 7.5, 25°C, inhibition of ATPase activity Hepacivirus C
3.6.1.15 3.5
-
 dGTP pH 7.5, 25°C, inhibition of ATPase activity Hepacivirus C
3.6.1.15 3.8
-
 ddCTP pH 7.5, 25°C, inhibition of ATPase activity Hepacivirus C