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Literature summary extracted from

  • Barabas, O.; Pongracz, V.; Kovari, J.; Wilmanns, M.; Vertessy, B.G.
    Structural insights into the catalytic mechanism of phosphate ester hydrolysis by dUTPase (2004), J. Biol. Chem., 279, 42907-42915.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.23 native and mutant enzyme Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.6.1.23 hanging-drop vapor diffusion method Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
3.6.1.23 D90N no effect on Km, strong decrease in catalytic activity, H-bonding significantly altered Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.23 0.45
-
 dUTP D90N mutant enzyme, pH 7.8, room temperature Escherichia coli
3.6.1.23 0.5
-
 dUTP wild-type enzyme, pH 7.8, room temperature Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.23 Mg2+ required Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.23 dUTP + H2O Escherichia coli removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA dUMP + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.23 Escherichia coli P06968
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.23 recombinant proteins Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.23 dUTP + H2O
-
 Escherichia coli dUMP + diphosphate
-
 ?
3.6.1.23 dUTP + H2O removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA Escherichia coli dUMP + diphosphate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.23 0.0003
-
 dUTP D90N mutant enzyme, pH 7.8, room temperature Escherichia coli
3.6.1.23 11
-
 dUTP wild-type enzyme, pH 7.8, room temperature Escherichia coli