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Literature summary extracted from

  • Sandu, C.; Chiribau, C.B.; Brandsch, R.
    Characterization of HdnoR, the transcriptional repressor of the 6-hydroxy-D-nicotine oxidase gene of Arthrobacter nicotinovorans pAO1, and its DNA-binding activity in response to L- and D-nicotine Derivatives (2003), J. Biol. Chem., 278, 51307-51315.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.99.4 gene 6hdno of pAO1, DNA sequence analysis, genetic organization, primer extension analysis, transcriptional analysis, binding and regulatory role of repressor HdnoR, effector protein IR1 is involved, overview Paenarthrobacter nicotinovorans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.99.4 additional information gene repressor HdnoR binds to the 6hdno gene operator, Kd is 21 nM, and represses enzyme expression, repressor in induced by 6-hydroxy-D-nicotine and 6-hydroxy-L-nicotine in vitro and in vivo Paenarthrobacter nicotinovorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.99.4 D-nicotine + acceptor + H2O Paenarthrobacter nicotinovorans isozyme specific for the D-isomer (R)-6-hydroxynicotine + reduced acceptor
-
 ?
1.5.99.4 L-nicotine + acceptor + H2O Paenarthrobacter nicotinovorans isozyme strictly specific for the L-isomer (S)-6-hydroxynicotine + reduced acceptor
-
 ?
1.5.99.4 additional information Paenarthrobacter nicotinovorans enzyme expression and activity is regulated by substrate and product level via transcription repressor HdnoR ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.99.4 Paenarthrobacter nicotinovorans Q59127 30 kDa; strain bearing the catabolic plasmid pAO1, 2 stereospecific isozymes, gene 6hdno encodes the D-nicotine inducible and specific isozyme, gene 6hlno encodes the L-nicotine inducible and specific isozyme
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1.5.99.4 Paenarthrobacter nicotinovorans Q59128 small 17.8 kDa subunit; strain bearing the catabolic plasmid pAO1, 2 stereospecific isozymes, gene 6hdno encodes the D-nicotine inducible and specific isozyme, gene 6hlno encodes the L-nicotine inducible and specific isozyme
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1.5.99.4 Paenarthrobacter nicotinovorans Q59129 87.7 kDa; strain bearing the catabolic plasmid pAO1, 2 stereospecific isozymes, gene 6hdno encodes the D-nicotine inducible and specific isozyme, gene 6hlno encodes the L-nicotine inducible and specific isozyme
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.99.4 culture condition:citrate-grown cell
-
 Paenarthrobacter nicotinovorans
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.99.4 D-nicotine + acceptor + H2O isozyme specific for the D-isomer Paenarthrobacter nicotinovorans (R)-6-hydroxynicotine + reduced acceptor
-
 ?
1.5.99.4 L-nicotine + acceptor + H2O isozyme strictly specific for the L-isomer Paenarthrobacter nicotinovorans (S)-6-hydroxynicotine + reduced acceptor
-
 ?
1.5.99.4 additional information enzyme expression and activity is regulated by substrate and product level via transcription repressor HdnoR Paenarthrobacter nicotinovorans ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.99.4 6-hydroxy-D-nicotine oxidase
-
 Paenarthrobacter nicotinovorans
1.5.99.4 6-hydroxy-L-nicotine oxidase
-
 Paenarthrobacter nicotinovorans

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.99.4 FAD both isozymes Paenarthrobacter nicotinovorans